道地药材平江白术挥发油的GC-MS指纹图谱研究  

Study on GC-MS fingerprint of essential oil from Atractylodes macrocephala Koidz. Produced in Pingjiang

作　　者：李然 李静[2] 童巧珍[3] 徐长琼 唐奇 夏丽琼 黄妍君 李旭富 黎春辉 LI Ran;LI Jing;TONG Qiaozhen;XU Changqiong;TANG Qi;XIA Liqiong;HUANG Yanjun;LI Xufu;LI Chunhui(Yueyang Hospital of Traditional Chinese Medicine,Yueyang 414000,China;School of Pharmaceutical Sciences,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China;School of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;Shantou University Medical College,Shantou 515041,China;Beijing Tongrentang Pingjiang Baishu Co.,Ltd.Yueyang 414000,China)

机构地区：[1]岳阳市中医医院,湖南岳阳414000 [2]广州中医药大学中药学院,广东广州510006 [3]湖南中医药大学药学院,湖南长沙410208 [4]汕头大学医学院,广东汕头515041 [5]北京同仁堂平江白术有限公司,湖南岳阳414000

出　　处：《广东药科大学学报》2019年第5期624-628,共5页Journal of Guangdong Pharmaceutical University

基　　金：湖南省自然科学基金科卫联合基金(2018JJ6081);湖南省科技厅中央引导地方科技发展专项资金(2019XF5069)

摘　　要：目的建立道地药材平江白术挥发油GC-MS指纹图谱的分析测定方法,为道地药材平江白术的质量控制提供参考。方法采用水蒸气蒸馏法提取白术挥发油,用GC-MS联用技术对平江白术进行指纹图谱测定,并运用中药色谱指纹谱图相似度评价系统进行分析。建立平江白术挥发油的共有指纹图谱,并进行相似度分析、聚类分析与主成分分析。结果建立了平江白术挥发油GC-MS指纹图谱,确定了17个共有峰;通过相似度分析、聚类分析、主成分分析结果可知,不同批次平江白术相似度较高,但质量存在一定差异。结论该方法操作性强、稳定与重复性好,可为平江白术的质量评价提供一定参考。Objective To establish a GC-MS fingerprint method for the analysis and determination of the essential oil of Atractylodes macrocephala Koidz.produced in Pingjiang,and to provide reference for the quality control of Atractylodes macrocephala Koidz.produced in Pingjiang.Methods The volatile oil of Atractylodes macrocephala Koidz.produced in Pingjiang was extracted by water vapor distillation,and the fingerprint of volatile oil was detected by GC-MS technique,and was calculated by the Traditional Chinese medicine fingerprint spectrum calculation software.GC-MS fingerprint of the volatile oil from Atractylodes macrocephala Koidz.produced in Pingjiang was established,and the methods of similarity analysis,clustering analysis and principal component analysis were used.Results GC-MS fingerprint of the volatile oil of Atractylodes macrocephala Koidz.produced in Pingjiang was established and 17 common peaks were identified.The results of similarity analysis,cluster analysis and principal component analysis showed that different batches of Atractylodes macrocephala Koidz.produced in Pingjiang had high similarity,but there were some differences in quality.Conclusion This method is operational,stable and reproducible,which can provide some reference for the quality evaluation of Atractylodes macrocephala Koidz.produced in Pingjiang.

关 键 词：平江 白术 挥发油 GC-MS 指纹图谱 

分 类 号：R284.2[医药卫生—中药学]