温度对HPLC法测定一清颗粒中黄芩苷的影响研究  

Study on Effect of Temperature on Determination of Baicalin in Yiqing Granules by HPLC

作　　者：魏志华[1] 黄小婉 段延鹏 王新民[1] Wei Zhihua;Huang Xiaowan;Duan Yanpeng;Wang Xinmin(Henan University of Animal Husbandry and Economy, Zhengzhou 450046;Zhengzhou University, Zhengzhou 450001, China)

机构地区：[1]河南牧业经济学院,河南郑州450046 [2]郑州大学,河南郑州450001

出　　处：《广东化工》2019年第19期172-173,194,共3页Guangdong Chemical Industry

基　　金：河南省教育厅重点项目(19B360002);河南牧业经济学院院级重点培育学科-中兽药生药学项目(41000003)

摘　　要：利用温度控制系统,改变HPLC分离柱的温度,进行测定一清颗粒中黄芩苷含量,结果表明,在完全相同流动相比例下,20~60℃范围内,HPLC系统压力、黄芩苷标准品以及一清颗粒样品中黄芩苷的保留时间、峰面积、有效塔板数、分离度等参数都发生了变化,在20~60℃之间一清颗粒样品中黄芩苷含量的测定值在45℃时最大,出峰数最多,有效塔板数和分离度均最大,图谱最优,黄芩苷峰较窄且对称性好。The content of baicalin in Yiqing granules was determined by HPLC using temperature control system and changing the temperature of HPLC separation column. The results showed that the HPLC system pressure, baicalin standard substance and retention time, peak area, effective plate number, separation degree and other parameters of baicalin in Yiqing Granules changed at the same ratio of mobile phase and at the temperature range of 20~60℃. It was at 45℃ that the determination value of baicalin content in Yiqing Granules, the peak number, the effective plate number and separation degree were all the largest, the chromatogram was the best, and the baicalin peak was narrow and symmetrical.

关 键 词：HPLC 温度 色谱峰 一清颗粒 黄芩苷 

分 类 号：TQ[化学工程]