抗酸化微生物复合菌系对餐厨垃圾堆肥腐殖质组分光谱学性质的影响  

Impact of Anti-Acidification Microbial Consortium on Spectral Characteristics of Humic Fractions in Food Waste Compost

作　　者：宋彩红 张亚丽[3] 李鸣晓 齐辉[1] 夏训峰 王丽君[2] 席北斗[2] SONG Cai-hong;ZHANG Ya-li;LI Ming-xiao;QI Hui;XIA Xun-feng;WANG Li-jun;XI Bei-dou(Life Science College,Liaocheng University,Liaocheng 252000,China;State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences, Beijing 100012,China;Institute of Geographic Sciences and Natural Resources Research,Beijing 100101,China)

机构地区：[1]聊城大学生命科学学院,山东聊城252000 [2]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [3]中国科学院地理科学与资源研究所,北京100101

出　　处：《光谱学与光谱分析》2019年第11期3533-3539,共7页Spectroscopy and Spectral Analysis

基　　金：国家水体污染控制与治理科技重大专项(2015ZX07103-007-03)资助

摘　　要：抗酸化微生物复合菌系(AAMC)通过多种耐酸、嗜酸微生物的协同作用,在克服由于酸化抑制导致的餐厨垃圾堆肥发酵崩溃问题方面效果显著,接种AAMC可明显加速有机物质降解。然而生物堆肥存在有机物彻底降解和碳重新固定(形成稳定的腐殖质类物质)两种途径,有机质降解与腐殖质形成具有互动关系,为腐殖质形成提供原材料。为探究接种AAMC对餐厨垃圾堆肥腐殖质品质的影响,采用树脂柱法进行腐殖质分组,分别研究接种AAMC对富里酸、亲水性组分和胡敏酸3个组分分子结构复杂度和稳定性的影响。设接种组(AAMC)、加碱组(MgO和K2HPO4)和自然堆肥组3个处理,采用三维荧光技术(EEM)结合两种定量表征方法区域体积积分(FRI)和平行因子分析(PARAFAC),实现对富里酸、亲水性组分和胡敏酸3个组分光谱学性质定量表征的准确性和完整性。FRI结果显示,堆肥结束后3个腐殖质组分中表征简单分子结构组分例如羧基或蛋白源结构区域的Pi,n值均降低,接种组降低幅度显著大于对照组,降低幅度大小排序为:接种组>加碱组>对照组。表征高芳香度和缩聚程度的胡敏酸类物质区域的Pi,n值均上升,且接种组上升幅度显著高于其他两处理,上升幅度排序也为:接种组>加碱组>对照组。PARAFAC结果显示,富里酸和胡敏酸组分又可分成短波长胡敏酸、长波长胡敏酸和色氨酸或类蛋白类物质3个组分,亲水性组分又可分为短波长胡敏酸、色氨酸和酪氨酸3个组分。堆肥结束后,表征短波胡敏酸和长波胡敏酸组分的Fmax升高,而表征色氨酸等类蛋白类物质组分的Fmax降低,升高或降低的幅度接种组最高,显著高于加碱组和对照组。综上结果说明接种AAMC可明显促进腐殖质组分子结构复杂化、稳定化,提高腐殖质组分高芳香度和缩聚程度,改善餐厨垃圾堆肥腐殖质品质,利于施用堆肥土壤保水保肥。这可能与AAMC具�Anti-acidification microbial consortium(AAMC)is effective in overcoming inhibition of microbial activity by acidification of composting material and avoiding the collapse of food waste composting under the synergistic action of acid-tolerant and acidophilic microorganisms.The degradation of organic substances could be accelerated by inoculation with AAMC.Complete degradation of organic matter and carbon refixation(the formation of humic substances)occur simultaneously during composting. Organic matter degradation has an interactive relationship with the formation of humic substances.The former provides raw materials for the latter.In order to explore the effect of inoculating anti-acidification microbial consortium (AAMC)on the quality of humic substances of food waste compost,the humic substances were grouped by resin column method and the influence of inoculating AAMC on molecular structure complexity and stability of the three fractions,such as fulvic acid,hydrophilic fraction and humic acid.In this study,Inoculation group(AAMC,Inoculation),alkaline compound treatment group(MgO and K2HPO4,AC)and natural composting group(C)were set.Three-dimensional fluorescence technology(EEM)was used to realize accurate and complete quantitative characterization of spectral properties of three humic fractions combined with two quantitative characterization methods FRI and PARAFAC.FRI results showed that the Pi,nvalues of those regions,which represented simple molecular structure components,such as carboxyl and protein source structure decreased in all three humic fractions after composting.The degree of reduction in the inoculation group was significantly greater than that in the control group and degree of reduction was in the order Inoculation>AC>C.The Pi,nvalues of those regions,which represented humic acid-like compounds with high aromaticity and polycondensation degree increased in all three humic fractions.The degree of increase in the inoculation group was significantly greater than that in the other two treatments and

关 键 词：堆肥 餐厨垃圾 抗酸化 腐殖质组分 平行因子分析 

分 类 号：S132[农业科学—农业基础科学]