大鼠脊髓损伤后直肠平滑肌的结构变化  

Structural changes of rectal smooth muscle after spinal cord injury in rats

作　　者：高涛 朱晓中[1] 鲍丙波 李星玮[1] 林俊卿 黄腾立 柴益民[1] 张长青[1] 郑宪友[1] GAO Tao;ZHU Xiao-zhong;BAO Bing-bo;LI Xing-wei;LIN Jun-qing;HUANG Teng-li;CHAI Yi-min;ZHANG Chang-qing;ZHENG Xian-you(Department of Orthopaedic Surgery, Shanghai Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China)

机构地区：[1]上海交通大学附属第六人民医院骨科

出　　处：《上海交通大学学报（医学版）》2019年第9期963-968,963,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金（81672144）;上海市教育委员会高峰高原学科建设计划(20161429)~~

摘　　要：目的·探讨大鼠脊髓损伤后直肠平滑肌结构的变化。方法·取成年雌性SD大鼠20只,随机均分为脊髓损伤组和正常对照组2组。脊髓损伤组于L5与L6腰间神经节之间完全离断脊髓,正常对照组不做处理。3个月后,采用活性炭悬浮液灌胃法测定肠道通过时间并记录24h排便量;随后取肛门近端约1cm处的直肠组织,行苏木精-伊红(H-E)染色、Masson染色以及免疫组织化学染色观察大鼠直肠平滑肌的变化。结果·与正常对照组比较,脊髓损伤组大鼠肠道通过时间显著延长,24h排便量显著减少(P=0.000)。H-E染色结果显示,脊髓损伤后大鼠直肠平滑肌细胞排列顺序紊乱;Masson染色结果显示,脊髓损伤后大鼠直肠平滑肌层呈纤维化改变;免疫组织化学染色结果显示,脊髓损伤后大鼠直肠平滑肌α-平滑肌肌动蛋白(αsmooth muscle actin,α-SMA)表达量下降。结论·脊髓损伤后,大鼠出现排便功能障碍,其直肠平滑肌结构亦发生显著变化,主要表现为平滑肌层萎缩变性、纤维化以及α-SMA表达量下降。Objective · To investigate the changes of rectal smooth muscle structure after spinal cord injury in rats. Methods · Twenty adult female Sprague-Dawley rats were randomly divided into two groups, i.e., spinal cord injury group and control group. In the spinal cord injury group, the spinal cord was completely disconnected between L5 and L6, and control group was not surgically treated. After 3 months, gastric instillation was used to measure the intestinal transit time with the administration of activated carbon suspension and the mass of fecal pellets within 24 h was recorded. Then the rectal tissue was taken at about 1 cm proximal to the anus. The changes of rat rectal smooth muscle were detected by hematoxylin-eosin (H-E) staining, Masson staining and immunohistochemistry staining. Results · Gastric instillation showed that compared with control group, the intestinal transit time of spinal cord injury group significantly prolonged, and the mass of fecal pellets within 24 h significantly reduced. H-E staining showed that the arrangement of rat rectal smooth muscle cells was disordered after spinal cord injury. Masson staining showed fibrotic changes in the rectal smooth muscle layer of rats after spinal cord injury. Immunohistochemistry staining showed that the expression of α smooth muscle actin (α-SMA) in rat rectal smooth muscle decreased after spinal cord injury. Conclusion · After spinal cord injury, rats present defecation dysfunction. The structure of rectal smooth muscle also changes significantly, mainly including smooth muscle atrophy, fibrosis and decreased α-SMA expression.

关 键 词：脊髓损伤 排便功能 直肠 平滑肌 

分 类 号：R651.2[医药卫生—外科学]