湘西黄牛Y-SNPs遗传多样性研究  

Genetic Diversity of Y-SNPs in Xiangxi Cattle

作　　者：蔡翠翠 宁庆庆 陈秋明 祝远魁 陈宁博[2] 陈宏[2] 雷初朝[2] CAI Cui-cui;NING Qing-qing;CHEN Qiu-ming;ZHU Yuan-kui;CHEN Ning-bo;CHEN Hong;LEI Chu-zhao(Guyuan Branch of Ningxia Academy of Agriculture and Forestry Sciences,Guyaun,Ningxia 756000;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100;Hunan Tianhua Industrial Corporation Co. Ltd.,Lianyuan,Hunan 417126)

机构地区：[1]宁夏农林科学院固原分院,宁夏固原756000 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]湖南天华实业有限公司,湖南涟源417126

出　　处：《中国牛业科学》2019年第5期34-36,共3页China Cattle Science

基　　金：宁夏自然科学基金项目(2019AAC03295);国家肉牛牦牛产业技术体系资助项目(CARS-37)

摘　　要：[目的]通过Y-SNP分子标记方法研究湘西黄牛的遗传多样性、群体遗传结构及父系起源。[方法]采用PCR扩增、测序与生物信息学方法,对24头湘西黄牛的2个Y-SNPs(UTY-19和ZFY-10)标记进行多态性分析。[结果]结果表明,湘西黄牛有Y1和Y3两种单倍型组,频率分别为12.5%和87.5%,表明湘西黄牛可能有普通牛和瘤牛2个父系起源。湘西黄牛的Y-SNP遗传多样度为0.2283±0.0978,表明湘西黄牛具有较低的父系遗传多样性,品种纯度较高。[结论]湘西黄牛的父系起源为瘤牛Y3单倍型组,其Y1单倍型组为国外肉牛杂交所致。[Objective] To analyze the paternal origin, genetic diversity and population genetic structure of Xiangxi cattle, this study used Y-SNPs molecular markers.[Method] The authors used PCR amplification, sequencing and bioinformatics methods, to carry out the polymorphism analysis of 24 Xiangxi cattle samples using two Y-SNPs markers (UTY-19 and ZFY-10).[Result] The results showed that there were two haplogroups of Y1 and Y3, with the frequencies of 12.5% and 87.5%, respectively, indicating that Xiangxi cattle might be two paternal origins of Bos taurus and Bos indicus. The genetic diversity of Y chromosome was 0.2283±0.0978, indicating that Xiangxi cattle had lower paternal genetic diversity and higher breed purity.[Conclusion] The paternal origin of Xiangxi cattle is zebu Y3 haplogroup, the Y1 haplogroup in Xiangxi cattle came from hybridization of foreign beef cattle breeds.

关 键 词：湘西黄牛 Y-SNPS 单倍型组 父系起源 

分 类 号：S823.81[农业科学—畜牧学] S823.94[农业科学—畜牧兽医]