PEI功能化碳纳米管作为药物载体的研究  

PEI functionalized carbon nanotubes as drug carriers

作　　者：金媛媛 吴淑恒 刘梁 JIN Yuan-yuan;WU Shu-heng;LIU Liang(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生物与制药工程学院

出　　处：《武汉轻工大学学报》2019年第5期40-44,共5页Journal of Wuhan Polytechnic University

基　　金：国家自然科学基金项目(21602166);武汉轻工大大学校立科研项目(2018Y13)

摘　　要：碳纳米管(CNT)因其独特的结构和理化性质,在许多领域受到越来越多的关注。以碳纳米管为基础材料开发新型药物载体,通过碱性条件下多巴胺(DA)自聚合反应将聚多巴胺(PDA)包裹在碳纳米管上,再经迈克尔加成反应得到聚乙烯亚胺(PEI)修饰的碳纳米管(CNT@PDA@PEI)。通过红外光谱和热重分析对CNT@PDA@PEI进行表征,并对载体的载药性能和释放性能以及细胞毒性和细胞摄取情况进行了研究。结果表明,经PDA和PEI修饰的CNT合成成功,CNT@PDA@PEI可以很好结合并释放盐酸阿霉素(DOX),且具有较低的细胞毒性,能负载DOX进入细胞内。综上,CNT@PDA@PEI有望发展为一种新型高效的药物转运载体。Carbon nanotubes (CNT) have attracted more and more attention in many fields due to their unique structure and physicochemical properties. In this paper,a novel drug carrier was developed based on carbon nanotubes. Dopamine was wrapped on carbon nanotubes through dopamine self-polymerization reaction under alkaline conditions,and PEI modified carbon nanotubes (CNT@PDA@PEI) were obtained through Michael addition reaction. CNT@PDA@ PEI30k was characterized by FTIR and thermogravimetric analysis. The drug carrying performance,release performance,cytotoxicity and cell uptake of the carrier were also studied. The results showed that the synthesis of CNT modified by PDA and PEI was successful,and CNT@PDA@PEI could bind and release DOX well. It also had low cytotoxicity,and could load HepG2 into cells. In conclusion,CNT@PDA@PEI is expected to be developed as a new and efficient drug transport carrier.

关 键 词：碳纳米管 阿霉素 药物载体 生物安全性 

分 类 号：TB339[一般工业技术—材料科学与工程]