叶酸靶向CdSe/CdS/ZnS荧光量子点对肝癌HepG2细胞的体外检测  

In Vitro Detection of Human Hepatocellular Carcinoma Cells HepG2 by Folic Acid Targeting CdSe/CdS/ZnS Fluorescent Quantum Dots

作　　者：曹艳娟[1] 石云峰[2] 刘明芳[1] 石继飞[1] CAO Yan-juan;SHI Yun-feng;LIU Ming-fang;SHI Ji-fei(School of Medical Technology,Baotou Medical College of Inner Mongolia University ofScience and Technology,Baotou 014040,Inner Mongolia,China;School of Chemistry and Chemical Engineering,Anyang Normal University,Anyang 455000,Henan,China)

机构地区：[1]内蒙古科技大学包头医学院医学技术学院,内蒙古包头014040 [2]安阳师范学院化学化工学院,河南安阳455000

出　　处：《韶关学院学报》2019年第9期65-69,共5页Journal of Shaoguan University

基　　金：秦文斌科技教育基金（BYJJ-QWB 201708）

摘　　要：通过连续的异质外延生长法合成了水溶性硒化镉/硫化镉/硫化锌(CdSe/CdS/ZnS)量子点(QDs),用叶酸(FA)对其表面进行修饰,从而使叶酸特异性结合的量子点更有价值.应用荧光光谱研究了不同激发波长的发光特性,用激光共聚焦荧光显微镜观察荧光量子点与肿瘤HepG2细胞的结合.实验结果表明,量子点的最大荧光峰位置与激发光的激发波长有关,该功能化量子点能够特异性识别肝癌细胞.The water-soluble quantum dots CdSe/CdS/ZnS were synthesized by continuous heteroepitaxial growth method, and the surface was modified with folic acid, so that the quantum dots specifically bound by folic acid were more valuable. The luminescence properties at different excitation wavelengths were studied by fluorescence spectroscopy. The binding of fluorescent quantum dots to HepG2 cell was observed by laser confocal fluorescence microscopy. The experimental results show that the maximum fluorescence peak position of the quantum dot is related to the excitation wavelength of the excitation light, and the functionalized quantum dot can specifically recognize the liver cancer cells.

关 键 词：量子点 叶酸 肿瘤细胞 

分 类 号：Q599[生物学—生物化学]