红鳍东方鲀伪雌鱼卵巢发育迟滞的调控机制  

Mechanisms of delayed ovarian development in pseudo-female Takifugu rubripes

作　　者：胡鹏[1,2,3] 柳淑芳 刘新富[1,2] 刘海金 刘圣聪[4] 庄志猛 HU Peng;LIU Shufang;LIU Xinfu;LIU Haijin;LIU Shengcong;ZHUANG Zhimeng(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China;Key Laboratory of Fish Conservation and Utilization in the Upper Research of the Yangtze River Sichuan province, Neijiang Normal University, Neijiang 641100, China;Dalian Tianzheng Industry Co., Ltd. Dalian 116000, China)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业农村部海洋渔业可持续发展重点实验室,山东青岛266071 [2]青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266071 [3]内江师范学院,长江上游鱼类资源保护与利用四川省重点实验室,四川内江641100 [4]大连天正实业有限公司,辽宁大连116000

出　　处：《中国水产科学》2019年第6期1105-1115,共11页Journal of Fishery Sciences of China

基　　金：山东省重大科技创新工程专项(2018SDKJ0302);山东省泰山学者建设工程专项

摘　　要：伪雌鱼的培育是红鳍东方鲀(Takifugu rubripes)全雄制种技术研发的关键环节之一,然而外源雌激素诱导获得的伪雌鱼表现出卵巢发育迟滞,降低了其育种价值和效率。为探讨红鳍东方鲀伪雌鱼卵巢发育迟滞的调控机制,本研究从孵化后20日龄开始,用10 μg/L 17 β-雌二醇(E2)浸泡红鳍东方鲀稚幼鱼,每天浸泡1次,每次2 h,至90日龄结束。在90、180和330日龄分别采集处理组(10 μg/L E2)遗传雄性幼鱼和对照组(0 μg/L E2)遗传雌性幼鱼,比较两组幼鱼性腺的组织学和形态学变化特征、下丘脑-垂体-性腺轴相关激素(FSH、LH、E2和17α, 20βOH-PROG)和基因(fshr、lhr、erα、erβ1、erβ2)及脂质积累相关基因(lpl和vldlr)的变化规律。结果显示: 10 μg/L E2可将遗传雄性幼鱼全部诱导为伪雌鱼,且伪雌鱼直至330日龄未二次反转为间性或者雄性,但其性腺系数、卵母细胞数量及卵黄生成前期的卵母细胞面积均显著小于对照雌鱼。此外, 90日龄伪雌鱼的lhr和pgr的表达量显著高于同期对照雌鱼,而17α, 20β-PROG的含量及fshr的表达量显著低于对照组;180日龄伪雌鱼的vldl表达量显著低于对照组;330日龄伪雌鱼的激素含量及基因表达量没有显著差异。综合分析伪雌鱼性腺发育的形态学、组织学和性腺轴相关激素及基因变化规律可见,足够浓度的外源E2能够诱导并维持伪雌鱼的卵巢特征,但E2浓度过高,一方面可能抑制fshr和vldlr基因的表达,从而影响脂质在卵黄生成早期卵母细胞中的积累,导致红鳍东方鲀伪雌鱼卵母细胞生长迟缓;另一方便,高浓度E2抑制伪雌鱼卵原细胞减数分裂的启动,是导致红鳍东方鲀伪雌鱼卵母细胞数量较少的原因之一。The tiger puffer (Takifugu rubripes), the most valuable teraodontiformes fish, is widely cultured in northern China. Because the testes of male tiger puffers are regarded as a delicacy, males usually have a much higher value than females. Therefore, the production of an all-male population holds considerable potential to improve the economic benefit of tiger puffers. The first step to product an all-male tiger puffer population is to induce sex reversal of males by exogenous estrogens and obtain pseudo-females. However, previous studies have indicated that the ovarian development of pseudo-females is delayed after the exposure, with small number and sizes of oocytes. To explore the regulation mechanisms underlying delayed ovarian development in pseudo-females, in this study, tiger puffers were exposed to 10 μg/L E2 for 2 h once a day from 20 to 90 days posting hatching (dph), and genetic male fish from the treatment group (10 μg/L E2) and genetic female fish from the control group (0 μg/L E2) were collected at 90, 180 and 330 dph, respectively. Then, changes in the histological and morphological features of gonads, hormones (FSH, LH, E2, and 17α, 20βOH-PROG), and genes (fshr, lhr, erα, erβ1, erβ2, and pgr) on the hypothalamus-pituitary-gonad axis, and genes (lpl and vldlr) involved in lipid accumulation were monitored. The results showed that 10 μg/L E2 was able to induce sex reversal in genetic males and obtain pseudo-females, and those pseudo-females were not reconverted into males or intersex at 330 dph. However, the gonadosomatic index, the oocyte number, and the area of previtellogenic oocyte in pseudo-females was significantly smaller than that of the control. Moreover, compared with control females at 90 dph, lower expression levels of fshr and lower levels of 17a, 20βOH-PROG, as well as higher expression levels of lhr and pgr, were detected in pseudo-females. At 180 dph, only the expression levels of vldlr were significantly lower in pseudo-females than in the control. At 330 dph, there was no s

关 键 词：红鳍东方鲀 雌激素诱导 伪雌鱼 卵巢发育 下丘脑–垂体–肝脏–性腺轴 

分 类 号：S92[农业科学—渔业资源]