急性铜胁迫对暗纹东方鲀组织铜积累、氧化应激、消化酶、组织病变及脂代谢相关基因表达的影响  

Effects of acute copper stress from copper accumulation, oxidative stress, digestive enzymes, tissue lesions, and gene expression related to lipid metabolism in Takifugu fasciatus

作　　者：王涛 王玮 陈同庆 夏小雨 缪金晗 张红燕 尹绍武 WANG Tao;WANG Wei;CHEN Tongqing;XIA Xiaoyu;MIAO Jinhan;ZHANG Hongyan;YIN Shaowu(College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China;College of Life Science, Nanjing Normal University, Nanjing 210023, China)

机构地区：[1]南京师范大学,海洋科学与工程学院,江苏南京210023 [2]南京师范大学,生命科学学院,江苏南京210023

出　　处：《中国水产科学》2019年第6期1144-1152,共9页Journal of Fishery Sciences of China

基　　金：江苏省自然科学基金青年基金(BK20180728);国家自然科学基金青年基金(31800436)

摘　　要：通过 96 h 急性毒性实验研究水体铜暴露对暗纹东方鲀(Takifugu fasciatus)生理生化及脂代谢相关基因表达的影响。实验设置对照组、0.1 mg/L 和 0.2 mg/L (96-h LC50) 3 个铜处理组。结果表明,铜在暗纹东方鲀肝、肌肉和全鱼中的积累量随着处理浓度的提高而提高。同等浓度处理下,铜的积累量为肝脏>全鱼>肌肉。随着铜处理浓度的提高,肝脏中丙二醛(MDA)含量及抗氧化酶活性[谷胱甘肽过氧化物酶(GSH-PX),总超氧化物歧化酶(SOD)和过氧化氢酶(CAT)]显著上升。急性铜暴露诱发肝脏血细胞沉积以及血窦扩张的症状。在鳃中,诱发上皮细胞增生,顶部棒状以及产生动脉瘤等症状。急性铜胁迫后,暗纹东方鲀肠道中的淀粉酶活性显著上升,但脂肪酶活性显著下降。在肝脏中,随着处理浓度的提高,淀粉酶、蛋白酶和脂肪酶活性显著降低。在 0.1 mg/L 处理组,脂肪合成相关基因(G6PD、6PGD、LPL、Fas 和 Acc)的表达量最高。但在 0.2 mg/L 处理组中,脂肪分解相关基因(HSL 和 CPT 1)的表达最高。急性铜胁迫后对转运因子 PPARα的影响不显著,但转运因子 PPAR γ的表达量显著上升。本实验表明铜对暗纹东方鲀生理生化指标及脂代谢相关基因的表达均产生显著影响,本研究为暗纹东方鲀养殖过程中铜的合理使用提供有益的指导价值,也为生产中更好地监控重金属污染提供一定的技术指标参考。The study was conducted to determine the effects of waterborne Cu exposure on physiological, bio- chemical, and gene expression related to lipid metabolism in Takifugu fasciatus via a 96 h acute toxicity experi- ment. In the present study, T. fasciatus were respectively exposed to 0 (control), 0.1 mg Cu/L, and 0.2 mg Cu/L (96-h LC50). The results showed that Cu accumulation in liver, muscle, and whole fish increased with increasing Cu concentration. The order of Cu accumulation was liver>whole body>muscle at the same Cu dose. With the increase in Cu concentration, malondialdehyde (MDA) content and activities of antioxidant enzymes [glutathione peroxidase (GSH-PX), total superoxide dismutase (SOD), and catalase (CAT)] increased in the liver. Acute Cu exposure induced blood cells deposition in veins and dilatation of sinusoids in the liver, and also induced epithelial hyperplasia, clubbed tips, and aneurysms in gills. In the gut, amylase activity increased significantly, yet the lipase activity decreased after acute Cu stress in T. fasciatus. In the liver, activities of amylase, protease, and lipase decreased significantly with the increase in Cu concentration. The expression of genes involved in lipogenesis (G6PD, 6PGD, LPL, Fas, and Acc) under 0.1 mg Cu/L exposure was the highest. However, the expression of genes involved in lipolysis (HSL and CPT 1) was the highest in the 0.2 mg Cu/L treatment group. Acute Cu stress had no significant influence on transfer factor PPAR α, but the expression of PPAR γ was significantly up-regulated relative to that of the control. This study revealed the significant effects of Cu exposure on physiological, bio- chemical, and gene expression related to lipid metabolism in T. fasciatus, which provides beneficial guidance for the rational use of Cu in the production process of T. fasciatus, and also provides beneficial references to technical indicators for better monitoring of heavy metal pollution in fish production.

关 键 词：暗纹东方鲀 铜 积累 氧化应激 消化酶 组织病变 脂代谢 

分 类 号：S94[农业科学—水产养殖]