TRFIA法检测HBV表面抗原最佳临界值的探讨  

Exploring the Optimum Critical Value of HBV Surface Antigen Detected by TRFIA

作　　者：张蕾[1] 杨宗树[2] 苏欣 莫少华 刘丙雨 卢香云[3] ZHANG Lei;YANG Zong-shu;SU Xin;MO Shao-hua;LIU Bing-yu;LU Xiang-yun(Department of Blood Transfution,the People's Hospital of Xinjiang Shihezi,Xinjiang Shihezi,832000;Department of Laboratory,the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi,832008;Shihezi University School of Medicine,Xinjiang Shihezi,832002)

机构地区：[1]新疆石河子市人民医院输血科,新疆石河子832000 [2]石河子大学医学院第一附属医院检验科,新疆石河子832008 [3]石河子大学医学院,新疆石河子832002

出　　处：《农垦医学》2019年第4期324-327,共4页Journal of Nongken Medicine

摘　　要：目的:探讨时间分辨荧光免疫分析法检测乙型肝炎病毒(HBV)表面抗原诊断阳性的最佳临界值。方法:选取某三甲医院住院或体检的170例经TRFIA法定量检测阳性的血清或血浆标本和40例接近临界值(阴性)标本(0.03-0.05IU/mL),采用化学发光微粒子免疫分析法确认其阴、阳性。绘制ROC曲线,确定最佳临界值。结果:在试剂说明给定的临界值下,TRFIA法检测结果与确证试验结果比较,有统计学差异(P<0.05),两试验结果一致性的Kappa值为0.673。TRFIA法检测HBV表面抗原诊断阳性的最佳临界值为0.080IU/mL,此时,TRFIA法检测结果与确证试验结果一致性的Kappa值为0.832。结论:不同实验室应根据自身情况,制定适合本实验室最佳临界值,能一定程度上减少漏诊率和误诊率。Objective: To explore the optimum critical value of TRFIA for detection of hepatitis B virus (HBV) surface antigen. Methods: 170 serum or plasma samples detected positive of HBV surface antigen by TRFIA and 40 negative specimens but close to the critical value(0.03~0.05IU/mL) from patients or people who did physical examination in a Top-grade hospital were chosen and chemiluminescence particle immunoassay was used to confirm them positive or negative. Determined the optimum critical value by drawing the ROC curve through SPSS19.0. Results: Under the given critical value specified by the reagent,there was statistical difference between the results of TRFIA and confirmation(P<0.05). While the consistency of two test results was the Kappa value 0.673. However, the optimum critical value of TRFIA for detection of HBV surface antigen positive diagnosis was 0.080IU/mL. At that time, the Kappa value of consistency between the results of TRFIA and the results of confirmation was 0.832. Conclusion: According to own laboratory conditions, different laboratories should establish the optimum threshold suitable for the laboratory. To some extent, it can reduce the rate of missed diagnosis and misdiagnosis rate.

关 键 词：时间分辨荧光免疫分析法 乙肝表面抗原 临界值 

分 类 号：R512.62[医药卫生—内科学]