唾液酸受体在大鼠肠黏膜微血管内皮细胞中的表达  

Expression of Sialic Scid receptors on rat intestinal mucosa microvascular endothelial cells

作　　者：唐玉玲 王昭华 霍彩云 郭晓桐 杨光辉 李颖鑫 王书扬 田海燕[1] 胡艳欣[1] TANG Yu-ling;WANG Zhao-hua;HUO Cai-yun;GUO Xiao-tong;YANG Guang-hui;LI Ying-xin;WANG Shu-yang;TIAN Hai-yan;HU Yan-xin(College of Vetrinary Medicine ,China Agricultural University ,Beijing 100193 ,China)

机构地区：[1]中国农业大学动物医学院

出　　处：《中国兽医杂志》2019年第6期7-8,13,I0003,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金面上项目(31772702)

摘　　要：肠黏膜微血管内皮细胞在肠道中广泛存在,为研究流感病毒唾液酸α2,3 半乳糖(SAα2,3Gal)和α2,6 半乳糖(SAα2,6Gal)两种受体在肠黏膜微血管内皮细胞表面的表达情况,本试验利用植物凝集素免疫荧光染色方法和流式细胞术分别进行了定性和定量分析。结果显示,大鼠肠黏膜微血管内皮细胞(RIM-MVECs)表面同时表达唾液酸α2,3-半乳糖和α2,6-半乳糖两种受体,即同时表达人流感病毒受体和禽流感病毒受体,并且唾液酸α2,6 半乳糖的表达量显著高于α2, 3半乳糖受体的表达量。该试验结果表明,RIM-MVECs 可以被人源和禽源流感病毒感染,且更易被人源流感病毒感染。Intestinal mucosa microvascular endothelial cells are widely distributed in the intestinal tract. To study the expression of influenza virus sialic acid receptor on the surface of intestinal mucosa microvascular endothelial cells,immunofluorescent staining and flow cytometry were used. The results showed that RIM - MVECs expressed α- 2,3 - and α- 2,6 - linked sialic acid recep- tors and the expression of SAa2,6Gal receptor on RIM - MVECs was higher than that of SAa2,3Gal receptor,suggesting that RIM - MVECs may be more susceptible to human influenza virus infection.

关 键 词：肠黏膜微血管内皮细胞 唾液酸受体 流感病毒 免疫荧光染色 流式细胞术 

分 类 号：S865.12[农业科学—野生动物驯养]