H5N1亚型禽流感病毒感染SPF雏鸡免疫器官变化  

Changes of cellular immunein immune organs of SPF chickens infected with H5N1 subtype avian influenza virus

作　　者：党盛源 刘超男[1] 高雪丽[1] 姜南 赵良友 吕晓萍[1] 郑世民[1] DANG Sheng-yuan;LIU Chao-nan;GAO Xue-li;JIANG Nan;ZHAO Liang-you;LYU Xiao-ping;ZHENG Shi-min(College of Veterinary Medicine ,Northeast Agricultural University ,Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine ,Harbin 150030 ,China;College of Life Science and Technology ,Dalian University ,Dalian 116622 ,China;Drug Safety Evaluation Center of Heilongjiang University of Chinese Medicine,Harbin 150040 ,China)

机构地区：[1]东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室,黑龙江哈尔滨150030 [2]大连大学生命科学与技术学院,辽宁大连116622 [3]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040

出　　处：《中国兽医杂志》2019年第6期14-17,21,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30371066)

摘　　要：为了探讨 H5N1 禽流感病毒(AIV)感染对 SPF 雏鸡免疫器官细胞免疫功能的影响。以7日龄SPF雏鸡为研究对象,应用组织化学技术结合病理学方法,通过T细胞数量、增殖功能、CD4+和CD8+亚型数量及其比值变化的检测,对 H5N1亚型AIV感染 SPF 雏鸡后,免疫器官细胞免疫变化进行了较系统研究。结果发现,H5N1亚型AIV感染SPF雏鸡后3~7d,其胸腺、脾脏、腔上囊无论是T细胞数量还是胸腺和脾脏T细胞对Con A的增殖功能,以及CD4+和 CD8+T细胞数均显著低于对照雏鸡。病毒感染雏鸡后1 ~ 5d,CD4+/CD8+T 细胞比值也显著低于对照雏鸡;AIV感染雏鸡全部发病,呈现典型AIV感染症状,死亡率为40%。表明 AIV感染所致雏鸡免疫器官细胞免疫功能低下,是AIV免疫致病机制的重要环节。To investigate the effect of H5N1 subtype avian influenza virus ( AIV) infection on immune function of immune organs of SPF chickens. 7 - day - old SPF chickens were selected as the research object. The number and proliferation function of T lym- phocytes,the number and ratio of CD4 + and CD8 + T lymphocytes subtypes were detected by histochemical technology and pathologi- cal methods. The results showed that in the H5N1 subtype AIV infected - SPF chickens both the number of T lymphocytes in thy- mus,spleen,and Bursa of Fabricius and the multiplication capacity of T lymphocytes to Con A in thymus and splenic,the number of CD4 + and CD8 + T lymphocytes were significantly lower than those in control chickens at 3 - 7 days post infection. The ratio of CD4 +/CD8 + T lymphocytes was lower than that in the control chickens at 1 - 5 days post infection. All AIV infected chickens had typical symptoms of AIV infection,and the mortality rate was 40%. The results suggest that the impaired cellular immune function low in immune organs of chickens with infected AIV is an important link of AIV immune pathogenesis.

关 键 词：SPF 雏鸡 H5N1 禽流感病毒 T 细胞数量及其增殖功能 CD4 +和 CD8 + T 细胞 

分 类 号：S852.33[农业科学—基础兽医学]