轮钟花组织培养的最佳外植体及培养基筛选  

Best Explant and Culture Medium Screening for Cyclocodon lancifolius

作　　者：祝久洁 吴汉竹 孙庆文 郭文凯 徐文芬 ZHU Jiujie;WU Hanzhu;SUN Qingwen;GUO Wenkai;XU Wenfen(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州中医药大学

出　　处：《贵州农业科学》2019年第10期105-109,共5页Guizhou Agricultural Sciences

基　　金：贵州省国内一流学科建设项目“喀斯特地区特色药材引种驯化、野生抚育与品质评价研究”[GNYL(2017)008];贵州省普通高等学校工程研究中心项目“贵州省中药民族药材种质资源保存及评价过程研究中心”[黔教合KY字(2017)018];贵州省优秀青年科技人才项目“贵州特色中药民族药资源研究”[黔科合平台人才(2019)5658]

摘　　要：建立轮钟花的组织培养方法,为轮钟花进一步开发利用提供依据,以轮钟花茎段、叶及种子为外植体,通过添加不同浓度的6-BA、NAA和2,4-D于MS或1/2MS培养基中,筛选轮钟花组织培养繁殖的最适培养基及最佳外植体。结果表明:诱导茎段、叶形成愈伤组织的最适培养基为1/2MS+1.0mg/L6-BA+1.0mg/L2,4-D,诱导率为80.0%;诱导种子形成愈伤组织的最适培养基为1/2MS+1.0mg/L6-BA+0.5mg/L2,4-D,诱导率为80.0%;诱导茎段愈伤组织继代培养的最适培养基为1/2MS+1.0mg/L6-BA+0.5mg/L2,4-D;种子愈伤组织继代培养的最适培养基为1/2MS+1.0mg/L6-BA+0.2mg/L2,4-D;诱导种子不定芽的最适培养基为MS+0.2mg/L6-BA+0.3mg/LNAA,分化率为80.0%;种子是诱导轮钟花不定芽的最佳外植体。In order to establish the tissue culture method of Cyclocodon lancifolius ,and also to provide the basis for the further development and utilization of C. lancifolius ,using stem segments,leaves and seeds as explants,different concentrations of 6-BA,NAA and 2,4-D were added to MS or 1/2MS medium to screen the optimum medium and explant for tissue culture and propagation. Results:The optimum medium for callus induction from stem segments and leaves was 1/2MS+1.0 mg/L 6-BA+1.0 mg/L 2,4-D ,and the induction rate was 80.0%;The optimum medium for inducing callus from seeds was 1/2MS+ 1.0 mg/L 6-BA+0.5 mg/L 2,4-D,and the induction rate was 80.0%. The optimum medium for stem callus subculture was 1/2MS+1.0 mg/L 6-BA+0.5 mg/L 2,4-D,and the optimum medium for seed callus subculture was 1/2MS+1.0 mg/L 6-BA+0.2 mg/L 2,4 D. The optimum medium for inducing seed adventitious buds was MS+0.2 mg/L 6-BA+0.3 mg/L NAA,and the differentiation rate was 80.0%,and the seed was the best explant to induce adventitious buds.

关 键 词：轮钟花 组织培养 外植体 愈伤组织 快速繁殖 

分 类 号：S567.2[农业科学—中草药栽培]