HPV16E7在宫颈癌组织中表达升高并促进宫颈癌细胞系HeLa和C33A的增殖  

HPV16E7 is up-regulated in cervical cancer tissues which promotes the proliferation of cervical cancer cell lines HeLa and C33A

作　　者：黄丽凤 梁夯 张立峡 杨臻 HUANG Li-feng;LIANG Hang;ZHANG Li-xia;YANG Zhen(Department of Clinical Laboratory, Zhongxin Ecocity Hospital, Tianjin Medical University, Tianjin 300467;Department of Clinical Laboratory,Tianjin Haibin People s Hospital, Tianjin 300280;Department of Clinical Laboratory, Tianjin Wuqing People s Hospital, Tianjin 301700;Department of Clinical Laboratory, Tianjin Medical University Cancer Hospital, Tianjin 300060, China)

机构地区：[1]天津医科大学中新生态城医院检验科,天津300467 [2]天津市海滨人民医院检验科,天津300280 [3]天津市武清区人民医院肿瘤科,天津301700 [4]天津医科大学肿瘤医院检验科,天津300060

出　　处：《基础医学与临床》2019年第11期1537-1542,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81803548)

摘　　要：目的研究乳头瘤病毒HPV16E7在宫颈癌中表达水平及其在宫颈癌细胞系He La和C33A中的作用机制。方法 RT-qPCR和免疫组织化学染色检测宫颈癌患者组织及其对照组中HPV16E7、CEA和CA125 mRNA和蛋白水平,并分析3者之间的关系。RT-qPCR和ELISA检测宫颈癌患者及其对照组血清中HPV16E7、CEA和CA125水平变化。Western blot、MTT法、集落形成实验、流式细胞计量术探究HPV16E7在HPV阴性细胞系C33A及HPV阳性细胞系He La中发挥的作用。结果 HPV16E7、CEA和CA125 mRNA及蛋白水平在宫颈癌患者组织和血清中是高表达的(P<0. 05)。HPV16E7与CEA和CA125具有正相关的关系(P<0. 05)。HPV16E7在C33A细胞中不表达,在He La细胞中高表达。HPV16E7能够促进宫颈癌细胞系C33A和He La的增殖能力,促进细胞周期进程,并能够抑制细胞凋亡。结论 HPV16E7能够促进宫颈癌细胞的恶性行为,其表达水平在宫颈癌患者血清和组织中显著升高,并与CEA和CA125呈正相关的关系。Objective To investigate the expression of HPV16E7 in cervical cancer and its mechanism in HeLa and C33A cell lines. Methods RT-qPCR and immuohistochemical staining assays were used to detect the expression level of HPV16E7, CEA and CA125 in cervical cancer tissues and to analyze the correlation of HPV16E7 and CEA, CA125. RT-qPCR and ELISA assay were used to detect the expression level of HPV16E7, CEA and CA125 in cervical cancer serum. Western blot, MTT, colony formation, cell cycle and cell apoptosis assay were used to investigate the role of HPV16E7 in C33A (HPV-) and HeLa (HPV+) cells. Results The mRNA and protein levels of HPV16E7, CEA and CA125 in cervical cancer tissues and serum were up-regulated( P <0.05). The correlation of HPV16E7 and CEA, CA125 was positively relevant( P <0.05). HPV16E7 was up-regulated in HeLa cells and no expression in C33A cells. HPV16E7 promoted proliferation, cell cycle progress and inhibited cell apoptosis in HeLa and C33A cells. Conclusions Up-regulated HPV16E7 promotes malignant behavior in cervical cancer, which is positively related to CEA and CA125.

关 键 词：HPV16E7 CEA CA125 宫颈癌 恶性行为 

分 类 号：R684[医药卫生—骨科学]