双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响  

Effects of Dihydroartemisinin on Oxidative Stress in Liver of Weaned Piglets Induced by Lipopolysaccharide

作　　者：赵永伟 牛玉[1] 何进田 季书立 张莉莉[1] 王超[1] 王恬[1] ZHAO Yongwei;NIU Yu;HE Jintian;JI Shuli;ZHANG Lili;WANG Chao;WANG Tian(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院

出　　处：《畜牧兽医学报》2019年第10期2139-2146,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2018YFD0501101)

摘　　要：为了研究双氢青蒿素(DHA)对断奶仔猪氧化应激的影响,通过脂多糖(LPS)诱导的方法建立了氧化应激模型。选用健康的断奶仔猪30头,随机分成对照组(CON)、模型组(LPS)和处理组(LD,LPS+DHA),每组10个重复。CON组和LPS组饲喂基础日粮,LD组饲喂基础日粮+80mg·kg^-1DHA。各组饲喂相应日粮21d。在宰前4h对LPS组和LD组按照体重腹腔注射100μg·kg^-1LPS,CON组注射相同剂量的无菌生理盐水。结果显示,与CON组相比,LPS组肝中丙二醛(MDA)、蛋白质羰基(PC)、8羟基脱氧鸟苷(8-OHdG)和过氧化氢(H2O2)含量显著升高(P<0.05)。同时,LPS组肝中总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活力和还原型谷胱甘肽(GSH)含量显著降低(P<0.05)。与LPS组相比,LD组中MDA、PC、8-OHdG和H2O2含量显著降低(P<0.05),CAT、T-AOC和GPx的活力显著提高(P<0.05)。另外,与CON组相比,LPS组肝中核因子E2相关因子2(Nrf2)和CAT的mRNA表达量以及Nrf2和血红素加氧酶1(HO-1)的蛋白表达量均显著降低(P<0.05)。DHA处理可显著(P<0.05)逆转LPS引起的上述变化。结果表明,DHA可能通过Nrf2/ARE信号通路的调节来缓解由LPS引起的断奶仔猪氧化应激,本研究可为畜牧生产中减少氧化应激提供一定的理论依据。In order to study the effects of dihydroartemisinin(DHA)on oxidative stress in weaned piglets,a model of oxidative stress was established by injection of lipopolysaccharide(LPS).Thirty healthy weaned piglets were randomly divided into control group(CON),model group(LPS)and treatment group(LD,LPS+DHA).Each group had 10 replicates.CON group and LPS group were fed with basal diet,LD group was fed with basal diet+80 mg·kg^-1 DHA.Each group was fed with corresponding diet for 21 days.LPS group and LD group were intraperitoneally injected with 100μg·kg^-1 LPS at 4 hours before slaughter.CON group was injected with the same dose of sterile saline.Compared with the CON group,the contents of malondialdehyde(MDA),protein carbonyl(PC),8-hydroxy-2-deoxyguanosine(8-OHdG)and hydrogen peroxide(H 2O 2)in the liver of LPS group were significantly increased(P<0.05).Meanwhile,the activities of total antioxidant capacity(T-AOC),catalase(CAT),glutathione peroxidase(GPx)and the contents of reduced glutathione(GSH)in the liver of LPS group were significantly decreased(P<0.05).Compared with LPS group,the contents of MDA,PC,8-OHdG and H2O2 in LD group decreased significantly(P<0.05),and the activities of CAT,T-AOC and GPx increased significantly(P<0.05).In addition,the expression of nuclear factor E2-related factor 2(Nrf 2)and CAT and the protein expression of Nrf2 and HO-1 in liver of LPS piglets were significantly lower than those of CON group(P<0.05).DHA treatment significantly reversed the above changes induced by LPS(P<0.05).The results showed that DHA could alleviate oxidative stress in weaned piglets induced by LPS by regulating Nrf2/ARE signaling pathway,which provided a theoretical basis for reducing oxidative stress in livestock production.

关 键 词：双氢青蒿素 肝 氧化应激 脂多糖 

分 类 号：S828.91[农业科学—畜牧学] S853.74[农业科学—畜牧兽医]