机构地区:[1]中国农业科学院饲料研究所,农业农村部饲料生物技术重点实验室,北京100081 [2]北京英惠尔生物技术有限公司,北京100083 [3]甘肃农业大学动物科学技术学院,兰州730070
出 处:《畜牧兽医学报》2019年第10期2156-2165,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA
基 金:国家重点研发计划课题(2017YFD0502001);北京英惠尔生物技术有限公司横向课题(RS201805)
摘 要:旨在研究高精料饲粮中添加不同水平酵母培养物(YC,yeast culture)对育肥湖羊营养物质表观消化率和瘤胃发酵参数的影响。本研究选取4~5月龄、平均体重50 kg的健康育肥湖羊公羊120只,按照平均体重一致的原则,随机分为4组,每组5个重复,每个重复6只羊。对照组饲喂不添加YC的基础饲粮(精粗比为7.5∶2.5),试验A、B、C组分别在基础饲粮中依次添加10、20、40 g·d^-1的YC。预试期7 d,正试期30 d。饲养试验结束时,每组选取5只羊进行消化代谢试验,测定各营养物质的表观消化率;采集瘤胃液,测定瘤胃发酵参数。结果表明:1)与对照组相比,A、B组有提高干物质采食量的趋势(P=0.064),A、B、C组饲粮DM和OM的表观消化率均显著高于对照组(P<0.05),但各添加剂量组之间差异不显著(P>0.05)。A、B组的CP表观消化率显著高于对照组(P<0.05),A组NDF、ADF的表观消化率显著高于对照组(P<0.05)。2)A、B、C组消化能和代谢能的摄入量显著高于对照组(P<0.05),并且A和B组的总能代谢率显著高于对照组(P<0.05)。3)A、B、C组的表观可消化氮显著高于对照组(P<0.05),但各添加剂量组之间差异不显著(P>0.05);各组间沉积氮、氮利用率和氮的生物学价值差异均不显著(P>0.05)。4)各组间瘤胃pH、总挥发性脂肪酸、乙酸、丙酸、丁酸及乙酸/丙酸等指标差异均不显著(P>0.05);A和C组的NH3-N浓度显著高于对照组(P<0.05)。以上结果表明,在本试验条件下,添加10 g·d^-1 YC提高了育肥湖羊的营养物质表观消化率,改善了能量和氮的利用率,但在改善瘤胃内环境方面无明显作用;饲喂更高剂量的YC没有表现出额外的作用效果。The objective of this study was to investigate the effects of different levels of yeast culture(YC)supplementation in high-concentration diet on nutrients apparent digestibility and rumen fermentation parameters of fattening Hu sheep.A total of 120 healthy male Hu sheep with an average body weight of 50 kg(4-5 months old)were randomly assigned into 4 groups(n=30)balanced on body weight.Sheep in control group(CON)were offered a basal diet(concentrate∶forage=75∶25)without YC supplementation,sheep in 3 experimental groups as group A,B and C were fed the basal diet supplemented with 10,20 and 40 g·d^-1 YC,respectively.Each group had 5 replicates with 6 sheep.The whole experiment lasted 37 d with an adaptation period(7 d)and experiment period(30 d).At the end of the experiment,5 sheep were selected from each group for digestive metabolism experiment for determination of apparent digestibility and rumen fermentation parameters.The results showed as follows:1)Group A and B had a tendency to increase dry matter intake(P=0.064);The apparent digestibility of DM and OM in group A,B and C were significantly higher than that in CON group(P<0.05),but there were no significant differences among group A,B and C(P>0.05);The apparent digestibility of CP in group A and B were significantly higher than that in CON group(P<0.05);In addition,the apparent digestibility of NDF and ADF in group A were significantly higher than that in CON group(P<0.05).2)GE and ME intakes in group A,B and C were significantly higher than that in CON group(P<0.05);The metabolizability of GE in group A and B were significantly higher than that in CON group(P<0.05).3)Apparent digestible nitrogen in group A,B and C were significantly higher than that in CON group(P<0.05),but there were no significant differences among group A,B and C(P>0.05);There were no significant differences in retained nitrogen,utilization rate and biological value of nitrogen among all groups(P>0.05).4)There were no significant differences in rumen pH,TVFA,acetate,propionate,butyr
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