HOXB7反义寡核苷酸对口腔鳞癌细胞迁移和运动能力的影响  

Effects of HOXB7 antisense oligonucleotides on migration and movement of oral squamous cell carcinoma

作　　者：邓晶[1] 邢育珍[1] DENG Jing;XING Yuzhen(Center of Stomatology, Union Medical College Affiliated to Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China)

机构地区：[1]华中科技大学同济医学院附属协和医院口腔医学中心

出　　处：《临床与病理杂志》2019年第10期2095-2101,共7页Journal of Clinical and Pathological Research

基　　金：湖北省自然科学基金(2016CFB670)~~

摘　　要：目的:探讨HOXB7反义寡核苷酸对口腔鳞癌细胞迁移和运动能力的影响。方法:在口腔鳞癌细胞系Tscca中转染HOXB7反义寡核苷酸和无义寡核苷酸,记为AS-ODN和NS-ODN,设置不转染寡核苷酸的细胞为Control,采用real-time PCR和蛋白质印迹法检测细胞中HOXB7表达变化,MTT测定细胞增殖和黏附能力变化,细胞划痕实验检测细胞运动能力,Transwell小室检测细胞侵袭和迁移能力,蛋白质印迹法测定细胞中E-cadherin,N-cadherin,MMP-2,MMP-9表达水平。结果: HOXB7反义寡核苷酸转染后细胞中HOXB7表达水平降低,细胞增殖、黏附、运动、侵袭和迁移能力均下降,细胞中N-cadherin,MMP-2,MMP-9蛋白水平降低,E-cadherin蛋白水平升高,与转染无义寡核苷酸的细胞比较,差异具有统计学意义(P<0.05)。结论: HOXB7反义寡核苷酸具有抗口腔鳞癌细胞增殖、黏附、运动、侵袭、迁移和上皮-间充质转化的作用。Objective: To investigate the effects of HOXB7 antisense oligonucleotides on migration and movement of oral squamous cell carcinoma. Methods: HOXB7 antisense oligonucleotides and nonsense oligonucleotides were transfected into oral squamous cell carcinoma cell line Tscca, named as AS-ODN and NS-ODN. The cells that did not transfect oligonucleotides was named as Control. Real-time PCR and Western blot were used to detect the expression of HOXB7 in cells. Cell proliferation and adhesion ability were detected by MTT. Cell scratch test was used to detect cell motility. Transwell was used to detect cell invasion and migration. Western blot was used to detect the expression levels of E-cadherin, N-cadherin, MMP-2 and MMP-9 in cells. Results: After transfection of HOXB7 antisense oligonucleotides, the expression level of HOXB7 and cell proliferation, adhesion, movement, invasion, migration decreased, the levels of N-cadherin, MMP-2 and MMP-9 in cells decreased, the level of E-cadherin protein increased, compared with the cells transfected with nonsense oligonucleotides, the difference were statistically significant(P<0.05). Conclusion: HOXB7 antisense oligonucleotides can inhibit the proliferation, adhesion, movement, invasion, migration and EMT of oral squamous cell carcinoma cells.

关 键 词：口腔鳞癌 HOXB7 迁移 运动 黏附 

分 类 号：R739.8[医药卫生—肿瘤]