亚精胺浸种缓解盐碱胁迫对苜蓿生长抑制的研究  

Alfalfa Seed Soaking with Spermidine to Alleviates the Inhibition of Alfalfa Growth under Soil Saline-alkaline Stress

作　　者：夏关雪莹 王冰[2] 屈琳俐 黄升财 张美娟 程宪国[2] XIA Guan-xue-ying;WANG Bing;QU Lin-li;HUANG Sheng-cai;ZHANG Mei-juan;CHENG Xian-guo(College of Land and Environment,Shenyang Agricultural University,Shenyang110866,China;Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Life Science,Shanxi Normal University,Linfen 041000,China)

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110866 [2]中国农业科学院农业资源与农业区划研究所,北京100081 [3]山西师范大学生命科学院,山西临汾041000

出　　处：《中国草地学报》2019年第5期17-23,共7页Chinese Journal of Grassland

基　　金：国家重点研发计划项目“东北苏打盐碱地生态治理关键技术研发与集成示范”(2016YFC0501203);“973”重大基础研究项目“草地生产力形成机理与调控途径”(2015CB150800)

摘　　要：采用0.5mmol/L、1mmol/L亚精胺浸泡处理东苜1号紫花苜蓿种子,温室盐碱土盆栽培养,测定苜蓿的发芽率、生物量、渗透势、可溶性糖、叶绿素、脯氨酸、丙二醛及根际表面钠钾离子流速响应,结果表明亚精胺浸种提高了盐碱胁迫下苜蓿的发芽率、生物量,促进可溶性糖、叶绿素、脯氨酸、钾离子积累,降低了钠离子和丙二醛含量,且以0.5mmol/L亚精胺浸种效果最佳。Salinity-alkalinity of soil is a major obstacle factor limiting plant’s growth and development.In this study,we soaked alfalfa seeds with spermidine and investigated the changes of phenotype and physiological metabolites of alfalfa under saline-alkaline stress to provide a scientific reference for adaptive planting of alfalfa in soils with salinity-alkalinity.In this experiment,two concentrations of spermidine in 0.5mmol/L and 1mmol/L were used to soak the seeds of Dongmu No.1 alfalfa,then the soaked seeds of alfalfa were transferred into the salinity-alkalinity soils,which sampled from the experimental station of Northeast Normal University in Baicheng,Jilin province,and further pot-cultured in greenhouse to measure the germination rates and biomass.The changes in the osmotic potentials,soluble sugar,chlorophyll,proline,and malondialdehyde (MDA) in alfalfa were also determined.The results showed that spermidine soaking significantly promoted the germination rates and biomass of alfalfa under salt-alkali stress,and increased the accumulation of soluble sugar,chlorophyll,proline,soluble sugar,chlorophyll,proline and K + ion in alfalfa,but significantly lowered the contents of Na + ion and malondialdehyde under salt-alkali stress.Soaking seeds with a solution of 0.5mmol/L spermidine revealed the best regulatory effect in enhancing salinity-alkalinity tolerance.Seed soaking with spermidine effectively improved the alfalfa’s growth and development under salt-alkali stress.Seed soaking with spermidine increased the accumulation of compatible metabolites in alfalfa leaves,and conferred a beneficial balance of intracellular osmotic potentials,thus reducing Na + the damage to the plant and leading to an enhanced salt-alkali tolerance in alfalfa.This study confirmed that the solution of 0.5mmol/L spermidine demonstrated the best effect in alleviating the salinity-alkalinity damage to alfalfa plants,indicating that application of exogenous spermidine could provide a signal regulatory role for maintaining alfalfa growth

关 键 词：苜蓿 亚精胺 盐碱胁迫 浸种 

分 类 号：Q945.78[生物学—植物学]