氟化钠对骨组织主要细胞类型的影响  

The effect of sodium fluoride on the main bone cell types

作　　者：蒋宁宁 郭奉杨 史立群 徐辉[1] JIANG Ningning;GUO Fengyang;SHI Liqun;XU Hui(School of Pharmaceutical Sciences,Jilin University,Changchun 130021;Alihe Community Health Service Center,Orochun Banner,Inner Mongolia Autonomous Region,Orochun Banner 165450,China)

机构地区：[1]吉林大学药学院再生医学科学研究所,吉林长春1300212 [2]内蒙古自治区鄂伦春自治旗阿里河社区卫生服务中心,内蒙古鄂伦春165450

出　　处：《中国骨质疏松杂志》2019年第10期1389-1392,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目(81673111)

摘　　要：目的明确氟化钠对骨组织3种细胞的活性作用特点。方法探究暴露于不同氟化钠浓度下的3种骨组织主要细胞活性的变化。小鼠骨髓间充质干细胞(bone marrow mesenchymal stemcells,BMSCs)作为成骨细胞祖细胞,通过含有地塞米松、抗坏血酸和β-甘油磷酸钠的矿化培养基诱导为成骨细胞。RAW264. 7细胞利用RANKL诱导其成为破骨细胞,而IDG-SW3细胞经过矿化诱导成为骨细胞。收集对数生长期的成骨细胞、破骨细胞和骨细胞,传至96孔板,施加给8个氟浓度梯度进行处理,包括对照组、低氟浓度(0. 1、0. 5、1、2 mg/L)、中氟浓度(4、8 mg/L)、高氟浓度(16、32 mg/L) 9个实验组。3种细胞分别经氟处理1、2、4 d后,采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞活性。结果染氟2 d后,0. 5 mg/L和1 mg/L氟显著提高了骨细胞的活性,但8~32 mg/L氟均显著抑制了破骨细胞和成骨细胞的活性。染氟4 d后,骨细胞的活性在0. 1~2mg/L氟处理下显著增高,1~4 mg/L氟处理下的破骨细胞的活性也明显比对照组高;高剂量氟16 mg/L处理下,骨细胞活性下降了8. 6%,而破骨细胞和成骨细胞活性则下降了90. 8%和97. 2%。结论氟对成骨细胞的刺激作用范围最窄,而抑制作用显著,破骨细胞对氟剂量的变化最敏感,而骨细胞对氟化物蓄积毒性的耐受性最强。Objective To clarify the viability trends of three types of bone cells exposed to sodium fluoride. Methods In order to explore the changes of cell viability on the three major bone cell types exposed to different concentrations of sodium fluoride, mouse bone mesenchymal stem cells(BMSCs), as osteoblast progenitors, were induced into osteoblasts by mineralization induction agents containing dexamethasone, ascorbic acid, and β-sodium glycerol phosphate. RAW264.7 cells were induced to become osteoclasts by RANKL induction, and IDG-SW3 cells were induced into osteocyte by mineralization induction. Osteoblasts, osteoclasts and osteocyte were seeded into 96-well plates and exposed to 8 fluoride concentration gradients, respectively. Fluoride gradient included control group, low fluoride concentration(0.1, 0.5, 1, 2 mg/L), medium fluoride concentration(4, 8 mg/L) and high fluoride concentration(16, 32 mg/L). Three types of cells were treated with fluorine for 1 day, 2 days and 4 days periods. In the end, the methyl thiazolyl tetrazolium(MTT) method was used to measure cell viability. Results After 2 days of fluoride exposure, 0.5 and 1 mg/L fluoride significantly increased the viability of osteocytes, but the 8-32 mg/L dose of fluorine significantly inhibited the viability of osteoclasts and osteoblasts. After 4 days of fluoride exposure, 0.1-2 mg/L fluoride treatment significantly increased the viability of osteocyte, the viability of osteoclasts treated with 1-4 mg/L fluoride was also significantly higher than that of the controls;Under treatment with 16 mg/L of fluoride, cell viability of osteocytes fell by 8.6%, while viability of osteoclast and osteoblast sharply fell by 90.8% and 97.2%. ConclusionBy comparison among three types of bone cell, osteoblasts indicated the narrowest range of fluoride-stimulating action, and significant inhibitory effect. Osteoclasts were the most sensitive to the change of fluorine dose, and osteocytes had the strongest tolerance to toxicity of fluoride accumulation.

关 键 词：破骨细胞 骨髓间充质干细胞 骨细胞 细胞活性 氟化钠 细胞类型 骨质疏松 

分 类 号：R599.9[医药卫生—内科学]