双波长吸光度法确定微量滴定板测试中稀释精确度的研究  

A Dual-wavelength Absorbance Method for Determining Dilution Accuracy in Microtiter Plate Assays

作　　者：许诚 张诚春 张怡雯 Xu Cheng;Zhang Chengchun;Zhang Yiwen

机构地区：[1]苏州市计量测试院

出　　处：《计量与测试技术》2019年第10期71-75,共5页Metrology & Measurement Technique

摘　　要：在许多基于微量滴定板的分析测试中,会经常涉及稀释的步骤。由于目标样品在溶液中的准确浓度通常不是直接测得的,而是根据稀释比计算得到,因此就需要准确测量每个稀释步骤,以便知道这些样品的最终浓度。本文介绍了一种基于双染料的双波长光度法,可以用于验证多步骤稀释中的每个稀释步骤。本文阐述了该方法的理论基础,并对该方法进行了实验验证。For many types of microtiter plate - based assays,dilution - based volume transfer steps are commonly performed.Because the exact concentrations of target samples in solution are typically not directly measured but calculated from the dilution ratio,it is important to accurately measure each dilution step so that the ultimate concentrations of those samples can be known.This paper describes a new approach,which is based on dual - dye,dual - wavelength photometry,for accurately measuring each dilution step in a multistep dilution protocol.The theory behind this method is described,as well as an experimental demonstration of the prescribed approach.

关 键 词：稀释实验 连续稀释 稀释精确度 双染料光度法 双波长光度法 

分 类 号：TB938.3[一般工业技术—计量学]