HCV核心区多肽对细胞毒T细胞作用的初步观察  

A preliminary study of effects of hepatitis C virus nucleopolypeptides on specific cytotoxic T cells

作　　者：马巧玉[1] 王宇明[1] 郝飞[1] 

机构地区：[1]第三军医大学西南医院感染病科,重庆400038

出　　处：《中华传染病杂志》2002年第5期294-296,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目 ( 3 9770 682 )

摘　　要：目的　探讨HCV感染者体内细胞毒T细胞 (CTL)功能缺陷的原因 ,为深入研究HCV感染的发病机制和指导疫苗研制提供理论和实验依据。方法　采用多肽固相合成法选择性合成HCV核心区多肽 ,并将其皮下注射免疫Balb/c小鼠 ,用乳酸脱氢酶 (LDH)释放实验检测小鼠脾细胞(CTL)活性。结果　经单因素方差分析显示 ,HCV核心区多肽CPA9( 39～ 74位氨基酸 )、CPB7( 6 7～ 76位氨基酸 )、CPB8( 71～ 80位氨基酸 )对小鼠CTL有抑制作用 ,CPA10 ( 5～ 2 3位氨基酸 )、CPB6 ( 6 3～ 72位氨基酸 )和CPB2 ( 131～ 14 0位氨基酸 )对小鼠CTL有增强作用。结论　经初步观察 。Objective To explore the mechanism of CTL dysfunction in HCV infected person, to provide a theoretical basis for further understanding the pathogenesis of hepatitis C and the development of HCV vaccines. Methods HCV nucleopolypeptides were selected and synthesized with the method of solid phase synthesis. BALB/c mice were immunized subcutaneously with HCV nucleopolypeptides, and CTL activity of mice was detected by LDH releasing test. Results CTL of mice could be inhibited by HCV nucleopolypeptides residues 39-74, 67-76, 71-80 and enhanced by HCV nucleopolypeptides residues 5 23,63 72,131 140. Conclusion The function of CTL can be suppressed and intensified by different HCV nucleopolypeptides.

关 键 词：HCV核心区多肽 T淋巴细胞 细胞毒性 

分 类 号：R512.63[医药卫生—内科学]