海带岩藻-半乳聚糖硫酸酯对体外培养的人外周血淋巴细胞的影响  

Effect of Fucoidan-Galactosan Sulfate from Laminaria japoni-ca on lymphocytes in human peripheral blood in vitro

作　　者：张英慧[1] 王海仁[2] 尹格平[3] 孙晓明[3] 

机构地区：[1]佛山科学技术学院北院农学系,广东南海528231 [2]山东大学生物化学与分子生物学系,山东济南250100 [3]山东省济南军区总医院,山东济南250031

出　　处：《中国海洋药物》2002年第5期10-12,共3页Chinese Journal of Marine Drugs

基　　金：国家九五科技攻关计划资助项目(项目编号:96-C01-05-02)

摘　　要：目的:检测海带岩藻-半乳聚糖硫酸酯（Fucoidan-Galactosan Sulfate, FGS）对体外培养的人外周血淋巴细胞增殖及亚群分化的影响。方法:本实验采用体外培养人外周血淋巴细胞的方法,并利用流式细胞仪检测FGS的药物活性。结果:FGS各剂量组（6.25～100μg·mL-1）中CD3+、CD4+、CD8+及CD19+、CD25+高于对照组,且CD4+/CD8+值也比对照组有所提高。结论:FGS可促进人外周血T、B细胞的增殖分化,提高IL-2R的含量。Objective To investigate the effect of Fucoidan-Galactosan Sulfate(FGS) which was isolated and purified from Laminaria japonica Aresch on lymphocytes in human peripheral blood in vitro. Methods Flow cytometry was used to observe the levels of CD3+ , CD4+ , CD8+, CD19+ and CD25+. Results We found that the levels of CD3+, CD4+, CD8+, CD19+ and CD25+in the FGS groups (6. 25-100μg/mL) were higher than that of control group, and the ratio of CD4+/CD8+ was also increased as compared with the normal. Conclusion FGS could stimulate the differentiation and proliferation of lymphocytes in human peripheral blood in vitro.

关 键 词：体外培养 海带岩藻-半乳聚糖硫酸酯 流式细胞术 淋巴细胞 亚群分化 

分 类 号：R282.77[医药卫生—中药学]