藻类脂肪酸分离提纯及检测  

Isolation, Purification and Assay of Algae's Fatty Acids

作　　者：苏秀榕[1] 娄永江[1] 李丹[2] 李哲[2] 康景轩[3] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211 [2]辽宁师范大学生命科学学院,辽宁大连116029 [3]美国哈佛大学医学院,ma02129

出　　处：《中国海洋药物》2002年第5期22-25,共4页Chinese Journal of Marine Drugs

基　　金：辽宁省教委重点资助项目(项目编号:20042099)

摘　　要：报道了藻类脂肪酸用索氏提取器提取脂肪、甲酯化后用气相色谱仪测定的方法。从单细胞藻类中提取脂肪酸必须破细胞壁后再用乙醚浸泡,提取的时间要达12h以上。大型藻类不用破壁,室温下用乙醚浸泡24h,再用索氏法提取5h左右即可。脂溶性色素含量较高的样品,用乙醚提取的脂肪要在检测之前用活性炭和硅藻土进行脱色,两种物质的量要适宜,否则将影响脂肪酸的含量。硅胶C-CMC混合薄层层析（正己烷:乙酸乙酯85.15为展层剂,10％磷相酸乙醇液为显色剂）,是快速检测脂肪酸的方法。计算脂肪酸的含量时,仅能计算加入石油醚和苯的上层体积,因为下层不含有脂肪酸。This paper reports the results of using the gas chromatography methods to analyze the algae's fatty acids after methylied, then purify and assay the fatty acids by Soshi isolator. The microalgae's cell wall must be breakdown before being immersed and extracted about 12h by ether. The kelp cell wall needn't be removed, its extraction time is about 5 hours by 24h pre-immersion with ether. The extraction liquid sample must be decolored by activated carbon or diatomaceous earth. It is the best method to assay fat acid with thin-layer chromatography made up with silica gel and carboxymethyl cellulose. The n-hexane and ethyl acetate (85 : 15) used as developing solvent, and 10% phosphomolybdic acid is a better chromogenic agent. The most of fatty acids dissolved in the supernatant consist of the petroleum ether and benzene, which volume of the subnatant needn't be calculated as fatty acids, because the sub-natant doesn't contain any fatty acids.

关 键 词：检测 单细胞藻类 多细胞藻类 脂肪酸检测 气相色谱 薄层层析 

分 类 号：R282.77[医药卫生—中药学]