5—单硝酸异山梨酯的致突变性研究  

Mutagenic Studies of Isosorbide 5-mononitrate (5-ISMN)

作　　者：戚亦宁 田秀杰[1] 金鑫[1] 卢亚利[1] 

机构地区：[1]北京市药品检验所药理毒理室,北京100035

出　　处：《癌变．畸变．突变》1992年第5期35-37,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文选用Ames试验、小鼠骨髓细胞微核试验和体外细胞染色体畸变试验,对5-单硝酸异山梨酯(5-ISMN)的致突变性进行了研究。Ames试验结果显示,在所选剂量范围内和±S9的条件下,5-ISMN对测试菌株的回复突变菌落数无明显影响,为致突变阴性。在微核试验中,各给药组微核发生率与阴性对照组相比,无显著性差异,P>0.05。染色体畸变试验中也未观察到5-ISMN对体外培养CHL细胞的染色体损伤作用。研究结果表明,5-ISMN对原核生物和哺乳动物细胞无致突变作用。Mutagenicity of 5-ISMN was studied using the salmonella/microsome assay, murine mi cronucleus assay and chromosome aberration assay. The mutagenic activity of 5-ISMN was not ob served in TA97a, TA98, TA100 and TA102 with and without S9 activation under the doses used. No significant differences in the number of micronucleated PCEs were found in any of the testing groups compared with negative control in mice, P>0. 05. In chromosome aberration test, the effect of chro mosomal damage of 5-ISMN was not found either in CHL cells. This studies indicated that 5-ISMN was not mutagenic in bacteria and mammalian cells.

关 键 词：硝酸酯 5-ISMN 致突变 

分 类 号：R994.1[医药卫生—毒理学]