湖羊蛋白酶遗传型结构及系统地位的研究  

Studies on Genotype Structure of Blood Protein and Enzyme and Phylogeny Status of Hu Sheep

作　　者：孙伟[1] 常洪[1] 杨章平[1] 耿荣庆[1] 倪大雄 范宝生 TsunodaK. 

机构地区：[1]扬州大学畜牧兽医学院,扬州225009 [2]浙江省湖州市畜牧兽医总站,湖州313000 [3]ShowaUniversity,SchoolofMedicine

出　　处：《中国农业大学学报》2002年第5期101-106,共6页Journal of China Agricultural University

基　　金：国家自然科学基金国际合作研究资助项目 (30 2 130 0 9)

摘　　要：用“中心产区典型群随机抽样”的方法 ,对湖羊品种进行遗传检测。在所检测的 12个位点中的 11个 (Alb、Tf、Alp、L ap、Ary- Es、Hb- β、X- p、CA、Cat、L y和 Ke)具有多态性 ,仅 Po位点为单态 ,多态座位比例为 91.6 7% ,在 2 7个等位基因中 ,只有 Po F、Tf A、Tf F、Hb- βA、CAF 和 Ke L 的估计可靠程度分别为 0 .5 2 2 2 ,0 .74 5 8,0 .5 2 2 2 ,0 .6 2 12 ,0 .8990和 0 .90 70外 ,其余 2 1个等位基因估计可靠程度均在 0 .95 45以上。说明“中心产区典型群随机抽样”在特定遗传背景下是一种有效的抽样方法。湖羊的平均杂合度比国内其他绵羊种群偏低 ,而与国外其他群体相比 ,平均纯合度偏低。引用国内外 14个绵羊群体的相同资料 ,根据血液酶和其他蛋白质变异的 10个基因座位共计 33个等位基因的频率 ,进行系统聚类分析 。Hu sheep is an indigenous breed in Zhejiang province of China.Based on the'central area typical colonies random sampling method,'the genotypic structure of blood protein and enzyme in Hu sheep was investigated. Of 12 loci, polymorphism was found at 11 loci, only Po locus was monomorphic.The proportion of polymorphic loci was 91.67%. The total number of allele was 27. Of the 27 alleles, the gene frequency reliability of gene frequency estimates for Po F, Tf A, Tf F, Hb β A, CA F and Ke L was 0.5222, 0.7458, 0.5222, 0.6212, 0.8990 and 0.9070, respectively, the others reached beyond 0.9545, which showed that'central area typical colonies random sampling method'is effective in the special genetic background. The average heterozygosity ( H ) and average homozygosity (J) were computed and the J of the sheep colonies in the countries around China in this study was found to be higher than that of Hu sheep. In order to understand the the phylogeny status of Hu sheep, we collect the data of 14 sheep colonies distributed in the East and South of Central Asia and use the data of the 10 loci 33 alleles to cluster by Ward's method. The result indicates that HusheepclustercloselywiththeMongoliasheep,whichisconformedwiththeknownhistory.

关 键 词：湖羊 蛋白酶 遗传型结构 系统地位 

分 类 号：S826.2[农业科学—畜牧学]