84K杨树耐盐基因转化研究  

Salt-resistant Gene Transformation to Poplar 84K

作　　者：樊军锋[1] 韩一凡[2] 李玲[2] 彭学贤[3] 李嘉瑞[4] 

机构地区：[1]西北农林科技大学林科院,陕西杨陵712100 [2]中国林业科学研究院,北京100091 [3]中国科学院微生物研究所,北京100080 [4]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《西北林学院学报》2002年第4期33-37,共5页Journal of Northwest Forestry University

基　　金：陕西省科技厅科技攻关项目 (2 0 0 1K0 1-G7)

摘　　要：在已建立了 84K杨叶片外植体再生系统的基础上 ,利用叶盘法首次开展了 84K杨双价耐盐基因 mtl D/gut D的转化研究 ,经诱导不定芽及诱导生根阶段卡那霉素 (选择性抗生素 )连续筛选 ,获得了 1 6株卡那霉素抗性转化植株。抗性植株经 PCR检测 ,有 4株呈阳性。耐盐实验表明 ,3株阳性植株抗 Na Cl能力比对照有不同程度提高。On the basis of establishment of leaf explant regeneration system of poplar 84K, transformation of mtlD/gutD gene to poplar 84k by means of leaf disc was for the first time undertaken. Through successive selection in the period of shoot and root induction under high lever kanamycin pressure, 16 kanamycin resistant regenerated plants was obtained, of which 4 was positive through PCR analysis. The salt resistant test of these 4 positive plants showed that 3 of them were enhanced in salt tolerance ability to some extent. PCR analysis and salt resistance test preliminarily indicated that the successful transformation had been made.

关 键 词：基因转化 84K 耐盐基因 卡那霉素 叶盘法 

分 类 号：S792.11[农业科学—林木遗传育种]