未成熟日本血吸虫卵抗原诱导宿主肉芽肿病变免疫调节机理的研究  

The Immunoregulation Mechanism of Granuloma Formation Induced by Immature Egg Antigen of Schistosoma japonicum

作　　者：郑葵阳[1] 李允鹤[2] 骆伟[2] 龚唯[2] 夏超明[2] 胡永德[2] 

机构地区：[1]徐州医学院寄生虫学教研室,江苏徐州221002 [2]苏州大学医学院寄生虫学教研室,江苏苏州215007

出　　处：《中国预防兽医学报》2002年第6期421-423,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：采用 4wk_SEA、5wk_SEA和 6wk_SEA免疫BALB/c小鼠 ,在攻击感染后 0～ 8周对其血清抗体水平、脾脏淋巴细胞表型进行动态观察。结果显示 ,4wk_SEA免疫鼠血清抗体水平 (ELISA法 )较 5wk_SEA和 6wk_SEA及对照鼠者高 1～ 3个稀释度 ,且用 4wk_SEA为抗原检测的特异性抗体水平也较 5wk_SEA和 6wk_SEA者高。应用流式细胞仪分析表明三期SEA免疫鼠脾脏CD4 + /CD8+ 淋巴细胞随攻击感染时间的后移而下降。提示 4wk_SEA的抗病免疫机理与其诱导出的短程特异性抗体的体液免疫和CD4 + /CD8+ 细胞抑制趋势的细胞免疫相关。The kinetic antibody levels and splenic T lymphocyte phenotypes in BALB/c mice immunized with 4wk_,5wk_and 6wk_SEA were detected during 0～8wks after challenge infection.The titres of the antibody of sera from mice immunized with 4wk_SEA were 1～3 times more than those in mice with 5wk_and 6wk_SEA and controls,and also the antibody levels tested by using 4wk_SEA were higher than those using 5wi_and 6wk_SEA.The percentage of splenic CD4 + and CD8 + T lymphocytes of mice immunized with the 3 SEA decreased from 4wks to 8wks post challenge infection.The primary results indicated that the immunoregulation mechanism of granuloma formation induced by mature egg antigen of Schistosoma japonicum would be associated with the humoral immune response mediated by the short_term specific antibody and the cell immune response characteristics of inhibited tendency of splenic CD4 +/CD8 + lymphocytes.

关 键 词：日本血吸虫 小鼠 虫卵抗原 肉芽肿病变 免疫调节机理 抗病免疫力 血吸虫病 

分 类 号：R532.21[医药卫生—内科学]