应用PCR技术检测鸭疫里默氏杆菌的研究  

Detection of Riemerella Anatipestifer by PCR

作　　者：胡清海[1] 刘晓文[1] 赵世华 张知良[1] 丁铲[1] 苗晋锋[1] 刘华雷[1] 赵东伟[1] 

机构地区：[1]江苏省家禽科学研究所,江苏扬州225003

出　　处：《中国预防兽医学报》2002年第6期471-473,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省"九五"农业科技攻关项目 ;编号 :BE9932 4

摘　　要：根据已发表的鸭疫里默氏杆菌 15型CVL110 / 89株编码 4 2_kDa主要外膜蛋白的基因序列设计并合成一对引物 ,建立PCR方法 ,对 7个血清型的鸭疫里默氏杆菌纯培养菌及野外病死鸭病料组织进行检测。结果表明 ,7个血清型的鸭疫里默氏杆菌纯培养菌DNA都可扩增出 80 9bp的DNA片段 ,而对照的 2株大肠杆菌和 1株沙门氏菌纯培养物DNA扩增结果为阴性 ;在对 2 4只不同鸭场病死鸭肝、脑的检测中 ,脑的检出率为 19/ 2 4 (高于细菌分离的 13/ 2 4 ) ,肝脏的检出率为 11/ 2 4 (高于细菌分离的 8/ 2 4 )。由此可见 ,建立的PCR技术可用于鸭疫里默氏杆菌的鉴定和快速诊断 (取脑组织 )。但此方法是否对其他血清型的鸭疫里默氏杆菌也适用 ,有待于收集这些血清型的菌株进行验证。A pair of primers were designed and synthesized,which complemented to some regions of the 42_kDa major antigenic outer protein ompA gene of Riemerella anatipestifer serotype 15 strain CVL110/89.A PCR technique was developed to detect 7 serotype R.anatipestifer strains which growed on P_L medium and samples from 24 different duck farms.The result showed that a 809 bp DNA fragment as it was expected was present in all detected R.anatipestifer strains,while 2 Escherichia coli isolates and one Salmonella isolate as control gave negative result.Of 24 duck from different farms,19/24 and 11/24 gave positive result from brain and liver tissues respectively by PCR,while by isolation they were 13/24 and 9/24.As above,the PCR method could be used to indentification and rapid diagnosis (brain)of R.anatipestifer (infection).Futher research should be done to prove its applicability in other serotype strains.

关 键 词：PCR技术 鸭疫里默氏杆菌 检测 鸭 血清型 

分 类 号：S858.32[农业科学—临床兽医学]