Cyanide Intoxication in Mice Through Different Routes and its Prophylaxis by α-Ketoglutarate  

Cyanide Intoxication in Mice Through Different Routes and its Prophylaxis by a-Ketoglutarate

作　　者：R.BHATTACHARYA R.VIJAYARAGHAVAN 

机构地区：[1]Division of Pharmacology and Toxicology, Defence Research and Development Establishment Gwalior-474002, M.P. India,Division of Pharmacology and Toxicology, Defence Research and Development Establishment Gwalior-474002, M.P. India

出　　处：《Biomedical and Environmental Sciences》1991年第4期452-459,共8页生物医学与环境科学（英文版）

摘　　要：Antagonising effects of α-ketoglutarate (α-KG) could be attributed to complexing of the reactive nucleophile (CN-) to form cyanohydrin in cyanide intoxication. However, an enormous protection obtained could not be delineated on account of possible in situ binding of α-KG given intraperitoneally (i.p.) in mice to cyanide administered through the same route. The present study was designed to see the efficacy of a-KG alone or in combination with sodium nitrite (SN) and/or sodium thiosulfate (STS) in male mice exposed to cyanide administered through subcutaneous (s.c.) or inhalation route. A technique for generation of hydrogen cyanide (HCN) is also discussed. On the basis of protection index (PI), defined here as the LD50 of cyanide in protected mice/LD50 of cyanide in unprotected mice and survival time, STS + α-KG regimen was equipotent to the conventional SN + STS regimen. This is further substantiated by effect of α-KG in reducing plasma cyanide levels. The efficacy of α-KG remains undeterred irrespective of the route of cyanide intoxication, while the magnitude of protection varies.Antagonising effects of α-ketoglutarate (α-KG) could be attributed to complexing of the reactive nucleophile (CN-) to form cyanohydrin in cyanide intoxication. However, an enormous protection obtained could not be delineated on account of possible in situ binding of α-KG given intraperitoneally (i.p.) in mice to cyanide administered through the same route. The present study was designed to see the efficacy of a-KG alone or in combination with sodium nitrite (SN) and/or sodium thiosulfate (STS) in male mice exposed to cyanide administered through subcutaneous (s.c.) or inhalation route. A technique for generation of hydrogen cyanide (HCN) is also discussed. On the basis of protection index (PI), defined here as the LD50 of cyanide in protected mice/LD50 of cyanide in unprotected mice and survival time, STS + α-KG regimen was equipotent to the conventional SN + STS regimen. This is further substantiated by effect of α-KG in reducing plasma cyanide levels. The efficacy of α-KG remains undeterred irrespective of the route of cyanide intoxication, while the magnitude of protection varies.

关 键 词：STS Cyanide Intoxication in Mice Through Different Routes and its Prophylaxis by Ketoglutarate 

分 类 号：R595[医药卫生—内科学]