钝顶螺旋藻DNA中G,C的质量分数的测定  

Study on the Percentage of G.C in DNA of Spirulina Platensis

作　　者：胡一兵[1] 胡军 吴菲 陈岚 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]恩施体育运动学校,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2002年第3期55-57,共3页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：湖北省教育厅重点资助项目 ( 93A0 68)

摘　　要：用Zarrouk培养基培养钝顶螺旋藻NS - 90 0 2 0 ,从中提取DNA ,然后在加热条件下 ,使其DNA变性 ,跟踪DNA的变性过程中的增色效应 ,得到螺旋藻的熔解温度 (Tm) ,根据经验公式 (G +C) % =2 .4 4 (Tm - 6 9.3)可以算出螺旋藻体内G ,C百分质量分数 。Spirulina platensis NS -90020 was cultivated in Zarrouk medium and its DNA was extracted and purified. Each absorption value was measured when the purified solution was heated at different temperatures. Then the percentage of G,C contained in DNA of spirulina platensis was calculated based on the equation of(G+C)%=2.44* (Tm-69.3) .This result can be used for further research on molecular biological characteristics of spirulina platensis.

关 键 词：质量分数 钝顶螺旋藻 DNA变性 增色效应 熔解温度 遗传特性 DNA结构 测定方法 

分 类 号：Q949.22[生物学—植物学]