表面等离子体谐振(SPR)生化分析仪的研制  

Development of Surface Plasmon Resonance (SPR) Biochemical Analysis Instrument

作　　者：崔大付[1] 李向明[1] 蔡浩原[1] 李亚亭[1] 郑自攀[1] 陈翔[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100080

出　　处：《现代科学仪器》2001年第6期34-38,共5页Modern Scientific Instruments

基　　金：国家自然科学基金No .699361 0 1No.39990 570的资助

摘　　要：表面等离子谐振 (SurfacePlasmonResonance ,SPR)生化分析仪是一种基于物理光学原理的新型生化分析系统 ,SPR技术作为一种表面反应的生物传感技术 ,具有很重要的生化分析能力 ,可用于无标记实时监测许多种类生物分子之间的反应。SPR生化分析技术已成为直接实时观测生物分子间相互作用的主导技术。近年来SPR传感技术受到重点研究和发展 ;SPR生化分析仪也已经商品化。在本文中详细介绍了自行研制的新型SPR 2 0 0 0型生化分析仪 ,仪器的主要性能是 :入射角扫描范围 40°～ 70°,测角精度优于 1× 10 -3 度 ,探测折射率范围 1.0 4～ 1.47,温度范围 4℃～ 6 0℃、检测灵敏度可达 10 -11M等等。利用该系统实时测试了 1gG GAHIgG的动态反应过程。实验证明该分析仪精度和灵敏度高 ,适用范围宽 ,并已提供多家使用 。The surface plasmon resonance (SPR) bio chemical analysis instrument based on the principle of physio optics is a new analysis system. The surface plasmon resonance technology, as a surface interacted method, has shown a great potential for biochemical analysis, allowing real time analysis of biomolecular interactions between many species without the use of any labeled molecules. So, the SPR technology has become a leading technology in the field of direct real time observation of the biomolecular interactions. In recent years the SPR sensor technology has been major development and applications. The SPR biochemical analysis instrument has been commercialized. In this paper a new SPR 2000 type biochemical analysis instrument has been presented. The SPR 2000 instrument main specification is incident angle scanning range 40°～70°, measurement angle accuracy 0.001°, refractive index range 1.04～1.47,temperature range 4℃～60℃, sensitivity 10 -11 M etc. By this instrument an immunoreaction of human immunoglobulin G (IgG) and goat anti human immunoglobulin G (GAH IgG) has been real time tested. The SPR 2000 has high accuracy and sensitivity, such as better commercial characterization by experiment demonstration.

关 键 词：生化分析仪 研制 表面等离子体谐振 生物传感器 生物分子相互作用 流通注射分析 

分 类 号：TH79[机械工程—仪器科学与技术] TP212.3[机械工程—精密仪器及机械]