日本血吸虫Dad1 cDNA和DNA序列的比较分析  

Comparison between cDNA sequence and DNA sequence of Dad1 from Schistosoma japonicum

作　　者：李焱[1] 吴忠道[2] 余新炳[2] 孟玮[2] 李晖婷[2] 

机构地区：[1]中山大学肿瘤防治中心研究所,广州510060 [2]中山大学寄生虫学教研室

出　　处：《中国人兽共患病杂志》2002年第6期46-48,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金 (NO3 0 0 70 683 ) ;广东省"2 11工程"学科建设项目 (NO98169);国家教育部博士点基金 (NO 2 0 0 0 45 )资助

摘　　要：目的　对日本血吸虫新基因Dad1进行深入分析。方法　抽提日本血吸虫成虫DNA做模板 ,根据SJDad1的开放读框序列设计一对引物进行扩增 ,将扩增产物克隆测序 ,应用BLAST2软件进行SJDad1的cDNA和DNA序列比较。结果　SJDad1的cDNA和DNA序列有一个碱基的差异 :距起始密码子ATG 339位cDNA该处是A ,而在DNA序列该处是G。结论　日本血吸虫Dad1不含内含子 ,cDNA和DNA序列的单碱基差异可能与RNA编辑有关。Aim To learn more about Schistosoma japonicum novel gene Dad1 Methods PCR was conducted while schistosome genomic DNA as template and a pair of primers designed according to Sj Dad1 ORF PCR products was cloned and sequenced Then cDNA sequence and DNA sequence of Dad1 was compared by using BLAST2 software Results Comparison between cDNA and DNA showed that only one nucleic acid was different:it was A in cDNA whereas G in DNA at 339 th base from ATG,the start codon Conclusion No intron in Sj Dad1 The single base difference between cDNA and DNA is possibly related to RNA editing

关 键 词：日本血吸虫 Dadl 序列分析 

分 类 号：R383.24[医药卫生—医学寄生虫学]