酸解——凝胶色谱法制备志贺菌O抗原多糖  

Method for preparation of OPS from Shigella by acetic acid hydrolysis and gel chromatography

作　　者：钟启平[1] 徐建设[1] 陈恩临[1] 

机构地区：[1]天津医科大学微生物学教研室,天津300070

出　　处：《中国人兽共患病杂志》2002年第6期58-60,共3页Chinese Journal of Zoonoses

摘　　要：本法在去除蛋白与核酸的条件下以 2 5 4nm作为脂多糖 (Lipopolysaccharide ,LPS)凝胶色谱检出波长 ,得到较高纯度志贺菌LPS。LPS经乙酸水解后 ,仍以 2 5 4nm作为O抗原多糖 (O -polysaccharide ,OPS)凝胶色谱的检出波长 ,可获得纯度优于常规方法且具较好免疫反应性的OPS ,该OPS与牛血清白蛋白 (bovineserumalbumin ,BSA)结合后具有较好免疫原性。We prepared the purified lipopolysaccharide(LPS)from Shigella flexneri 5 M90T by enzymatic hydrolysis and Sephadex G50 chromatography Subsequently the O polysaccharide(OPS)was isolated from the LPS by acetic acid hydrolysis,and was purified by gel chromatography with 254nm ultraviolet monitor The OPS reacted with the antiserum to M90T The OPS BSA conjugate efficiently induce specific antibodies against whole M90T bacterial cell

关 键 词：志贺菌 脂多糖 O抗原多糖 OPS 细菌性痢疾 免疫学 酸解-凝胶色谱法 

分 类 号：R516.4[医药卫生—内科学]