SjR47抗原免疫对血吸虫感染小鼠免疫细胞骨架超微结构影响的观察  

Observations on SjR47 antigen induced effects of ultrastructural changes of immune cells' skeleton in mice-infected with Schistosoma japonicum

作　　者：骆伟[1] 龚唯[1] 李立红 李允鹤[1] 庞智[1] 夏超明[1] 胡永德[1] 

机构地区：[1]苏州大学医学院寄生虫学教研室,苏州215007

出　　处：《中国人兽共患病杂志》2002年第6期61-63,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目 (编号 :3 9470 63 9)

摘　　要：目的　探讨日本血吸虫卵源性 4 7kDa组分抗原 (SjR4 7抗原 )对血吸虫感染小鼠腹腔巨噬细胞 (M)和脾淋巴细胞骨架的保护性效应。方法　应用扫描电镜 (SEM )和免疫电镜 (IEM)观察SjR4 7抗原免疫小鼠体内上述两种细胞骨架超微结构 ,并以感染和正常对照组小鼠作对比。结果　SjR4 7抗原免疫小鼠 ,攻击感染后 10w ,用SEM观察结果 ,两种免疫细胞骨架 ,均与正常对照组鼠的基本相似 ,但与感染对照鼠比较 ,后者的两种免疫细胞绒毛状结构几乎消失 ,显色反应低于其它两组。结论　SjR4 7抗原对日本血吸虫感染小鼠体内免疫细胞骨架超微结构能诱导明显的保护作用 ,使感染小鼠免疫细胞骨架避免或减少受损 ,其分子机制可能与Th1和Th2型细胞因子应答的调节效应相关。Aim In order to investigate the schitosome egg original 47kDa fractional antigen(SjR47 antigen)induced protective regulator effects on ultrastructural changes of peritoneal macrophages(M) and splenic lymphocytes' skeleton in schistosoma japonicum infected mice Methods The peritoneal M and splenic lymphocytes were observed by using high resolution scanning electron microscopy(SEM)and immune electron microscopy(IEM)in the experimental groups at the 11th week(10 weeks postinfection)after the mice had been immunized with SjR47 antigen(group Ⅰ),infected with schistosome cercariae only(group Ⅱ),and group Ⅲ were used as the normal mice control Results The fluffoid structure of both cells surface in group Ⅰ were similar to those in group Ⅲ,but shorter and in disorder a little,and those of the infection control group were almost disappeared In group Ⅰ,the positive reaction products of immune enzyme presented high electron density,thick grain and arranged lineally on the cell membrane,organellae and nuclear membrane The ultrastructural observations of both cells' skeleton of group Ⅰ were similar to those in group Ⅲ,those in group Ⅱ were more significantly changed than those in group Ⅰ and group Ⅲ Conclusion In this study,SjR47 fractional antigen can induce protective regurlator effects on ultrastructural changes of both immune cells' skeleton in Schistosoma japonicum infected mice and make the peritoneal M and spenic lymphocytes of host avoid or reduce the serious damage,the molecular mechanisms may be closely associated with regulator effects of Th1 type and Th2 type cytokines responses

关 键 词：日本血吸虫 SjR47抗原 免疫细胞骨架 超微结构 保护性调节效应 动物实验 

分 类 号：R532.21[医药卫生—内科学]