荧光波长对共焦显微镜成像特性的影响  

Influence of Fluorescent Wavelength on Imaging Property of Confocal Microscopy

作　　者：裴红津[1] 唐志列[1] 杨初平[1] 梁瑞生[1] 汪洁[1] 徐险峰[1] 

机构地区：[1]华南师范大学物理系,广州510631

出　　处：《光学学报》2002年第10期1219-1223,共5页Acta Optica Sinica

基　　金：广东省自然科学基金 (980 0 4 6 )资助课题

摘　　要：导出了共焦显微镜中不同荧光波长情况下的荧光功率传输函数、三维脉冲响应函数 (3D PSF)和三维光学传递函数 (3D OTF)。结果表明 ,不同的荧光波长对共焦显微镜的空间截止频率、分辨率、光学传递函数存在明显的影响。当激发波长与荧光波长的比值下降到一定程度时 。Fluorescence power transfer function, three-dimensional point spread function (3D-PSF) and three-dimensional optical transfer function (3D-OTF) for the various fluorescent wavelength are calculated. The results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut-off frequency, resolution and 3D-OTF. There is a missing-cone in the 3-D space of OTF when the ratio of excitation wavelength to fluorescent wavelength decreases.

关 键 词：荧光波长 共焦显微镜 成像特性 失锥现象 

分 类 号：TH742.65[机械工程—光学工程]