DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with ^(153)Sm-EDTMP  

作　　者：ZHU Shoupeng XIAO Dong HAN Xiaofeng (Suzhou medical College, Suzhou 215007) 

出　　处：《Nuclear Science and Techniques》1999年第1期5-7,共3页核技术（英文）

基　　金：the International Atomic Energy Agency

摘　　要：The apoptosis in bone tumor cells is studied after 153Sm-EDTMP irradiation. Fragmented DNA is analyzed by agarose gel electrophoresis. Experimental observations show that 153Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is shown. At the same time, the microautoradiographic study indicates that 153Sm-EDTMP could permeate through cell membrane and displays membrane-seeking condensation in bone tumor cells. Soon afterwards 153Sm-EDTMP could be phagocytized by the tumor cells and distributed ill cytoplasm as well as nucleus in the form of phagosome. With the prolongation of observing time, the membrane-bounded apoptotic bodies are observed.The apoptosis in bone tumor cells is studied after 153Sm-EDTMP irradiation. Fragmented DNA is analyzed by agarose gel electrophoresis. Experimental observations show that 153Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is shown. At the same time, the microautoradiographic study indicates that 153Sm-EDTMP could permeate through cell membrane and displays membrane-seeking condensation in bone tumor cells. Soon afterwards 153Sm-EDTMP could be phagocytized by the tumor cells and distributed ill cytoplasm as well as nucleus in the form of phagosome. With the prolongation of observing time, the membrane-bounded apoptotic bodies are observed.

关 键 词：DNA 骨癌细胞 153Sm-EDTMP辐照 

分 类 号：R817.8[医药卫生—影像医学与核医学] R738.1[医药卫生—放射医学]