重组海葵溶细胞素对大鼠血管平滑肌细胞增殖的影响  

Effects of recombinant sea anemone cytotoxin on vascular smooth muscle cell proliferation in rats

作　　者：欧阳平[1] 姜孝玉[2] 杨文利[2] 徐安龙[2] 

机构地区：[1]第一军医大学南方医院心内科,广东广州510515 [2]中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室,广东广州510275

出　　处：《第一军医大学学报》2002年第11期994-995,1002,共3页Journal of First Military Medical University

基　　金：国家高技术发展计划项目(2001AA62-8090);广州市科委科技攻关项目(JB00000448165)

摘　　要：目的观察重组海葵溶细胞素(Src)对离体大鼠血管平滑肌细胞增殖的影响。方法体外培养大鼠胸主动脉血管平滑肌细胞(VCMC),应用不同浓度的Src分别进行刺激VCMC并设立对照进行比较,采用非放射性的MTS/PES法确定血管平滑肌细胞的增殖状态。结果各组的细胞增殖率分别为对照组:0.802±0.055;Src100μg/ml组:0.115±0.014;Src10μg/ml组:0.213±0.016;Src1μg/ml组:0.335±0.097,Src100ng/ml组:0.663±0.060;Src10ng/ml组:0.678±0.129;Src1ng/ml组:0.738±0.072。统计分析显示100ng/ml以上浓度的Src能明显抑制大鼠血管平滑肌细胞的增殖(P<0.05),并呈现剂量依赖性(P<0.05)。结论重组海葵溶细胞素Src对大鼠血管平滑肌细胞增殖有一定的抑制作用。Objective To obs er ve the effects of recombined sea anemone cytotoxin (rSAC) on vascular smooth mus cle cell proliferation in rats rSAC. Methods Aortic smooth muscle cells (ASMCs) of rats were cultured in vitro and stimulated with rSAC of different concentra tions, and were observed in comparison with the control group. The ratio of cell proliferation was determined with non-radioactive MTS/PES assay. Results The r atio of cell proliferation rate was 0.802±0.055 in the control group, and was 0.115±0.014, 0.213±0.016, 0.335±0.097, 0.663±0.060, 0.678±0.129, and 0.738 ±0.072 in rSAC groups corre-sponding to rSAC concentrations of 100, 10, 1 mg/ml and 100, 10, 1 ng/ml respectively. It was shown that rSAC significantly inhibi ted the cell proliferation when the concentration used was above 100 ng/mg (P<0. 05), in a dose-dependent manner as com-pared with the control group (P<0.05). Co nclusions rSAC can significantly inhibit the vascular smooth muscle cell prolife ra-tion in rats.

关 键 词：血管平滑肌细胞 药物作用 重组海葵溶细胞素 细胞增殖 VCMC 

分 类 号：R285.5[医药卫生—中药学]