广东广西地区5个群体9个STR基因座的频率调查  

A genetic study of 9 STR loci in 5 populations living in Guangdong and Guangxi provinces

作　　者：刘超[1] 刘长晖[1] 朱少建 汪萍 李发贵 石朝清 

机构地区：[1]广州市刑事科学技术研究所,广东广州510030 [2]广西壮族自治区刑事科学技术研究所,广西南宁530012 [3]龙胜县公安局,广西龙胜541000

出　　处：《中国法医学杂志》2002年第5期263-266,共4页Chinese Journal of Forensic Medicine

基　　金：公安部科技攻关项目基金资助(编号 20004421401)

摘　　要：目的 调查广东汉族、广西汉族、广西侗族、广西壮族、广西苗族5个群体9个STR基因座多态性,探讨其在法医学检验中的应用价值。方法 应用AmpFISTR Profiler PlusTM荧光标记复合扩增系统,对广东广西5个群体4个民族的1191个无关个体的血样DNA进行9个STR基因座的复合扩增;用ABI 3100遗传分析仪对扩增产物进行分型,统计9个STR基因座的群体遗传学参数。结果 9个STR基因座在广东广西地区5个群体中的累积偶合率为1.51×10-11～8.08×10-11,累积非父排除率为0.99981—0.99990。,结论 该9个STR基因座可满足汉族、壮族、侗族、苗族群体法医学的个体识别及亲权鉴定的需要。Objective To investigate the genetic polymorphisms of 9 STR loci in Guangdong Han population, Guangxi Han population, Dong population, Zhuang population, and Miao population. Method DNA samples from 328 unrelated individuals in Guangdong Han population and Guangxi Han Population, 199 Zhuang popultion, 162 Dong population, 194 Miao population were screened by using AmpFISTR Profiler PlusTM PCR Amplification Kit and 3100 Genetic Analyzer. Results The genotype frequencies of these 9 STR loci meet the Hardy - Weinberg expectations.The matching probabilities of these 9 STR loci were between 1.51 x 10-11- 8.08 x 10-11 .The cumulative exclusion chances of paternity were 0.99981 - 0.99990. Conclusion Our results showed that the The Profiler Plus?PCR Amplification systems of 9 STR loci are useful in forensic case work in Guangdong Han population, Guangxi Han population, Dong population,Zhuang population and Miao population.

关 键 词：广东 广西 法科学 STR基因座 复合扩增 PCR 遗传多态性 

分 类 号：D919[医药卫生—法医学]