痛稳素和痛稳素(10～17)对孤啡肽对抗内吗啡肽-1及内吗啡肽-2镇痛作用的影响  

EFFECTS OF NOCISTATIN AND NOCISTATIN (10～17) ON NOCICEPTIN IN ANTAGONIZING ENDOMORPHIN-1 AND ENDOMORPHIN-2 ANALGESIA IN MICE

作　　者：陈鲤翔[1] 陈勇[1] 彭雅丽[1] 王转子[1] 陈强[1] 王锐[1] 

机构地区：[1]兰州大学生命科学学院生物化学与分子生物学系,甘肃兰州730000

出　　处：《药学学报》2002年第11期837-840,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金 (2 0 0 72 0 14 ) ;教育部高等学校优秀青年教师教学科研奖励计划资助项目 (第二届 )

摘　　要：目的　研究痛稳素及其碳末端八肽在小鼠脑内对孤啡肽对抗内吗啡肽 1及内吗啡肽 2镇痛作用的影响。方法　以固相多肽合成法合成了痛稳素及其碳末端八肽 ,侧脑室注射孤啡肽、痛稳素及其碳末端八肽 ,用辐射热甩尾法测定痛阈。结果　孤啡肽可对抗内吗啡肽 1及内吗啡肽 2的镇痛作用 ;痛稳素及其碳末端八肽不影响小鼠的基础痛阈 ,但可逆转孤啡肽对抗内吗啡肽 1及内吗啡肽 2的镇痛作用。结论　痛稳素及其碳末端八肽在脊髓以上水平可逆转孤啡肽对抗内吗啡肽 1及内吗啡肽 2的镇痛作用。AIM To investigate the effects of nocistatin and nocistatin (10～17) on nociceptin in antagonizing endomorphin-1 or endomorphin-2 analgesia in mice. METHODS Nocistatin and nocistatin (10～17) were synthesized by solid-phase peptide synthesis method. The mouse tail-flick test was used to assess the pain threshold of the mice and observe the effects of nocistatin and nocistatin (10～17). RESULTS Nociceptin was shown to antagonize endomorphin-1 and endomorphin-2 analgesia. Nocistatin or nocistatin (10～17) produced no significant effect on basic pain threshold; however,both of them were found to reverse the effects of nociceptin in antagonizing endomorphin-1 and endomorphin-2 analgesia. CONCLUSION Nocistatin and nocistatin (10～17) were shown to reverse the effects of nociceptin in antagonizing endomorphin-1 and endomorphin-2 analgesia at supraspinal level.

关 键 词：痛稳素 孤啡肽 内吗啡肽-1 内吗啡肽-2 镇痛作用 

分 类 号：R965[医药卫生—药理学] R971.1[医药卫生—药学]