铽敏化化学发光测定盐酸芦氟沙星  

DETERMINATION OF RUFLOXACIN HYDROCHLORIDE BY TERBIUM SENSITIZED CHEMILUMINESCENCE

作　　者：陆燕[1] 赵慧春[1] 孙春燕[1] 易琳[1] 

机构地区：[1]北京师范大学化学系

出　　处：《药学学报》2002年第11期876-879,共4页Acta Pharmaceutica Sinica

基　　金：国家自然基金资助项目 (2 99710 0 5 );北京市自然科学基金项目 (2 0 2 2 0 0 7) .

摘　　要：目的　研究Tb(III) RFX Ce4 + Na2 SO3体系的化学发光机理 ,建立测定胶囊和人体尿样中芦氟沙星含量的新方法。方法　将芦氟沙星注入到Na2 SO3载液中 ,按先后顺序与Tb3+ 和Ce4 + 溶液混合 ,用化学发光检测器测量体系的相对发光强度。结果　芦氟沙星的浓度在 2 0× 10 - 9～ 1 0× 10 - 6 mol·L- 1 ,与化学发光强度呈良好的线性关系 ;检出限为 3 8× 10 - 1 0 mol·L- 1 。结论　为芦氟沙星药代动力学研究和临床治疗提供一种高灵敏度、高选择性、简单快速的新方法。AIM To study the chemiluminescence mechanism of Tb(III)-RFX-Ce(IV)-Na_2SO_3 system and establish a new method for the determination of rufloxacin (RFX) in capsules and human urine samples. METHODS RFX was injected into Na_2SO_3 standard solution,then mixed with Tb~ 3+, Ce~ 4+ in turn, the chemiluminescence signal was detected by weak luminescence analyzer. RESULTS The chemiluminescence intensity was linearly related to the concentration of RFX in the range of 2.0×10~ -9～1.0×10~ -6 mol·L~ -1 with a detection limit of 3.8×10~ -10 mol·L~ -1. The familiar excipients such as starch and dextrine do not cause interference. So, this method can be applied to the determination of RFX in tablet. The content and recovery of RFX in urine can be directly detected without pretreatment. CONCLUSION It provides a simple, rapid, highly sensitive and highly selective method of rufloxacin for pharmacokinetic studies and clinical treatments.

关 键 词：芦氟沙星 铽 敏化化学发光 流动注射 测定 

分 类 号：R917[医药卫生—药物分析学]