肝素钠精制工艺研究  

Study on Purification of Heparin Sodium

作　　者：张万忠[1] 张辉[1] 李文秀[1] 毛睿[2] 马润宇[3] 

机构地区：[1]沈阳化工学院,辽宁沈阳110021 [2]沈阳药业股份有限公司,辽宁沈阳110043 [3]北京化工大学,北京100029

出　　处：《沈阳化工学院学报》2002年第3期191-194,共4页Journal of Shenyang Institute of Chemical Technolgy

摘　　要：采用过氧化氢二次氧化法精制肝素 .在该过程中采用低温离心技术 ,解决在除酸性蛋白过程中 ,沉淀不易滤除的困难 .并通过实验确立酶解结合氧化法精制肝素新工艺 ,克服粗品肝素钠中残留杂蛋白在纯化过程中很难被除去的缺点 .在粗品肝素钠中加入胰蛋白酶对这些杂蛋白进行酶解 ,再结合氧化除杂过程 。The purification of heparin by double oxidation process with H 2O 2 was investigated. The centrifugal technique under low temperature was used to solve the difficulty of removing of the precipitation. The the crude heparin sodium salt was degraded by adding trypsin, so that the residual proteins were removed from heparin. The results showed that the improved process, which combined oxidation and degradation, can raise the potency and recovery of heparin sodium salt.

关 键 词：肝素钠 精制 工艺 研究 过氧化氢 胰蛋白酶 

分 类 号：TQ464.54[化学工程—制药化工]