人重组生长激素对豚鼠心室肌细胞动作电位钙通道的作用  

Effect of Recombinant Human Growth Hormone on Action Potential of Ven- tricular Muscle Cell and Calcium Channel of Guinea Pigs

作　　者：孟繁波[1] 杨萍[1] 赵林阳[1] 周方君[1] 辛暨华[2] 许建春[3] 谭艳华 邹洪霞 

机构地区：[1]吉林大学中日联谊医院,长春130031 [2]长春生物制品研究所 [3]吉林中心医院 [4]吉林市医院 [5]朝阳区疾病预防控制中心

出　　处：《中国生物制品学杂志》2002年第6期350-352,共3页Chinese Journal of Biologicals

基　　金：吉林省科委资助;吉科合字第990581-2

摘　　要：目的 探讨人重组生长激素正性肌力作用的机制。方法 利用Langendorff模型以及膜片钳技术研究心肌细胞内向钙电流。结果 受到人重组生长激素作用10min后,动作电位平台期延长,APD50、APD90分别由261.14±15.42ms至354.65±31.22ms和311.23±13.7至401.33±18.13ms。最大内向峰电流(Ica)由 2.03±0.09升至2.85±0.11PA,增加约40％。结论 人重组生长激素延长动作电位平台期,促进正常豚鼠心肌细胞L-Ca2+通道开放。Objective To explore the effect of recombinant human hormone on action potential of ventricular muscle cell and calcium channel. Methods Study the action potential and influx calcium ion current of ventricular muscle cells of guinea pigs by using Langendorff model and patch - clamp technique. Results Ten minutes after the administration of recombinant human growth hormone, the plateau of action potential prolonged significantly. The APD50 and APD90 increased from 261. 14 ± 15.42 and 311. 23 ± 13. 7ms to 354.65 ± 31.22 and 401.33 ± 18.13ms respectively. The peak calcium ion current increased by about 40% , from 2.03 ± 0.09 to 2.85 ± 0.11 PA. Conclusion Recombinant human growth hormone prolonged the plateau of action potential and promoted the opening of calcium channel.

关 键 词：人重组生长激素 豚鼠 心室肌细胞 动作电位 钙通道 作用 

分 类 号：R977.13[医药卫生—药品]