碱蓬 c DNA表达文库的构建(英文)  

Construction of a cDNA expression library of Suaeda salsa

作　　者：马秀灵[1] 王丽萍[1] 张慧[1] 

机构地区：[1]山东师范大学逆境植物重点实验室,济南250014

出　　处：《西北植物学报》2002年第6期1289-1294,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究专项资金资助项目 ( G19990 1170 0 )

摘　　要：以盐生植物盐地碱蓬 (Suaeda salsa)地上部分为材料 ,提取植物总 RNA,纯化出 m R-NA,合成 c DNA第一链 ,得到双链 c DNA后 ,连接入植物表达载体 ,构建 c DNA表达文库。该文库重组子约 1 0 6。将该文库转化农杆菌 GV31 0 1 ,可直接用以转化拟南芥。In order to find new genes related to salt stress,we constructed a Suaeda salsa aerial part tissue cDNA expression library.Halophyte S.salsa was used as plant material,the first strands of cDNAs were synthesized from mRNA.Ligated the cDNAs into the expression vector,then transformed Escherichia.coli .The library titer is about 10\+6.Plasmids were extracted from amplified library and were transferred into Agrobacterium ,which subsequently was used to transform Arabidopsis thaliana .

关 键 词：CDNA表达文库 表达载体 盐地碱蓬 耐盐性 抗逆性 

分 类 号：Q943[生物学—植物学] S332[农业科学—作物遗传育种]