RAPD技术在芦荟属植物分类研究中的应用  

Studies on identification and classification of genomic DNA in Aloe by RAPD analysis

作　　者：李妮亚[1] 高培元 周鹏[3] 郭安平[1] 钟琼芯[1] 

机构地区：[1]海南师范学院生物系,海口571158 [2]海南热带植物组织培养研究中心,海口570314 [3]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101

出　　处：《西北植物学报》2002年第6期1432-1437,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：海南师范学院自然科学基金资助项目

摘　　要：采用 CTAB法提取 1 1个芦荟材料的基因组 DNA为模板 ,以 50个随机引物进行RAPD分析。结果表明 :大部分引物可以在不同模板上扩增出条带 ,但仅有 6个引物可以同时在 1 1个芦荟材料的 DNA上扩增出条带 ,对 1 1个芦荟种或变种的 RAPD结果进行聚类分析 ,结果表明基本符合传统分类观点。对 RAPD技术在芦荟属植物分类研究中的问题进行了讨论。The genome DNA of the 11 Aloe accessions was used as templates and amplified with 50 random primers by using RAPD,which was successfully extracted from the leaves of eleven Aloe accessions (including species,some varieties) by using the improved CTAB method.Results were shown that fragments were amplified on different templates with most of the random primers,but fragments were amplified from all templates only with six primers.According to the results of RAPD,the genetic relationship among the 11 accessions was analysed for classification of Aloe was discussed.

关 键 词：RAPD技术 芦荟属植物 分类研究 应用 

分 类 号：Q949.718.2[生物学—植物学]