转抗虫基因欧美黑杨离体快繁技术研究  

In vitro micropropagation of insect-resistant transgenic Populus euramericana

作　　者：康冰[1] 曹建军[1] 张小红[1] 李军超[1] 齐兰兰[1] 孙建钊[1] 桂兰芳[1] 董秀花[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2002年第6期1474-1478,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：以抗虫欧美黑杨的叶、带腋芽茎段为外植体进行离体快繁技术研究。最佳接种时间为8月份 ,新芽生长迅速。基本培养基为 MS,较适初培养基为 MS+6- BA0 .5mg/L(以下单位同 ) +NAA 0 .0 1 mg/L ,附加 30 g/L蔗糖、7g/L琼脂。愈伤组织诱导并同时分化出新芽培养基为 MS+6- BA 1 .5+NAA 0 .3,附加 40 g/L蔗糖、6 g/L琼脂。继代增殖培养基为 MS +6-BA1 .0 +NAA0 .1 +GA2 .0 ,附加 30 g/L蔗糖、5g/L琼脂。生根培养基为 MS+IBA2 .0。Shoots and axillary buds were induced in vitro from apexes,stem segments and leaves of insect-resistant transgenic Populus euramericana .August is the best inoculated time and new buds grew rapidly.Basic medium is MS medium.The proper initial culture medium is MS+6 BA 0.5 mg/L+NAA 0.01 mg/L,buds were regenerated from the explants.Calluses were induced and meanwhile cluster adventitious buds were formed on the MS basic containing 1.5 mg/L 6 BA and 0.3 mg/L NAA,high sugar concentration (40 mg/L) is suitable for calluses inducing and new buds forming.The medium for multiplication is MS+6 BA 1.0 mg/L+NAA 0.1 mg/L+GA 2.0 mg/L.The rooting medium is MS+IBA 2.0 mg/L.

关 键 词：转抗虫基因欧美黑杨 离体快繁技术 新芽增殖 生根培养 

分 类 号：S792.11[农业科学—林木遗传育种] S723.132[农业科学—林学]