牙鲆淋巴囊肿病的PCR诊断方法研究  

The Diagnostic Method of PCR for Lymphocystis Disease (LCD) of Cultured Paralichthys olivaceus

作　　者：刘允坤[1] 孙修勤[1] 黄捷[2] 张进兴[1] 

机构地区：[1]国家海洋局第一海洋研究所,青岛266061 [2]中国水产科学院黄海水产研究所,青岛266071

出　　处：《高技术通讯》2002年第11期87-89,共3页Chinese High Technology Letters

基　　金：863计划 (863 819 Z 0 6;2 0 0 1AA62 2 0 3 0 )资助项目

摘　　要：以中国养殖牙鲆 (Paralichthysolivaceus)淋巴囊肿病毒 (Lymphocystisdiseasevirus,LCDVcn)主要衣壳蛋白 (majorcapsidprotein ,MCP)基因的中间保守序列为目标基因 ,设计了一对特异性引物。该引物可扩增出 172bp的病毒DNA片段 ,其最小DNA检出量为 0 0 183ng。用PCR法从人工感染淋巴囊肿病毒 3天的牙鲆血、鳃、肝、脾、肠、胃及自然发病牙鲆的肿瘤中 ,分别检测到了LCDV的存在。本实验结果证明 ,PCR法对于早期检测LCDV是十分有效的。A pair of specific primers was designed based on the major capsid protein( MCP ) of lymphocystis disease virus (LCDVcn) in Paralichthys olivaceus. The part conservative sequence of the MCP was chosen as target gene. 172bp virus DNA fragment can be expressed from the primers by PCR, and the minimum detect limitation is 0.0183ng. LCDV has been detected by PCR method from the blood, gill, liver, spleen, intestines and stomach of Paralichthys olivaceus which has been artificially infected for 3days, and from the tumor of the spontaneously diseased Paralichthys olivaceus. The results showed that PCR method is effective in early detecting of LCDV. iseesedmum detect limitation is 0.0183n

关 键 词：牙PING 诊断方法 淋巴囊肿病毒 PCR 鱼病诊断 人工感染 衣壳蛋白 

分 类 号：S943[农业科学—水产养殖] S941.41[农业科学—水产科学]