反相高效液相色谱法测定龙血竭中紫檀(艹氐)的含量  

RP - HPLC Studies on Quantitative Determination of Pterostilbene in Dragon's Blood

作　　者：胡迎庆[1] 宗宁[1] 刘岱琳[1] 

机构地区：[1]中国人民武装警察部队医学院,天津300162

出　　处：《药物分析杂志》2002年第6期428-430,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(30070088)

摘　　要：目的:建立反相高效液相色谱法分离和测定龙血竭中紫檀芪的含量。方法:以Phenomenex C_(18)反相键合硅胶色谱柱(250 mm×4.6 mm,5μm),乙腈-1％冰醋酸(41:59)为流动相,流速1.5 mL·min^(-1),检测波长为310 nm,灵敏度为0.02 AUFS;柱温40℃;用外标法定量。结果:紫檀芪在41.6～208 ng范围有良好线性关系,r=0.999 5,理论塔板数以紫檀芪计为5 422;方法回收率为97.82％。结论:本方法是测定龙血竭中紫檀芪含量的准确、可靠、简便的定量方法。Objective: To establish a HPLC method for the separation and determination of pterostilbene in Drag-on's Blood. Methods: Using Phenomenex C18 reverse phase column (250 mm × 4. 6 mm, 5 μm) and acetoni-trile - 1% acetic acid (41 = 59) as mobile phase, flow rate of 1. 5 mL · min-1 , detecting at 310 nm and the sensitivity at 0.02 AUFS, the temperature of column was 40 ℃ and quantitating with external standard method. Results: The standard curve of pterostilbene was linear in the concentration range of 41. 6 -208 ng ( r = 0. 999 5). The theoretical plate number was 5 422. The average recovery was 97. 82% . Conclusion: The established method was accurate, sensitive, simple and reliable for determination of pterostilbene in Dragon's Blood.

关 键 词：反相高效液相色谱法 龙血竭 紫檀Qi 含量测定 

分 类 号：R286.0[医药卫生—中药学]