多聚酶链式反应扩增日本血吸虫基因组DNA重复序列  

PCR Amplification for Repetitive DNA Sequences from Genome of Schistosoma Japonicum

作　　者：何纳[1] 姜庆五[1] 赵根明[1] 刘建翔[1] 韦建国[1] 赵春琳[1] Joseph Hamburger 

机构地区：[1]复旦大学公共卫生学院流行病学教研室,上海200032 [2]以色列希伯来大学Kuvin传染病与热带病研究中心

出　　处：《中国公共卫生》2002年第11期1284-1285,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助 (项目批准号 :39870 655)

摘　　要：目的　探索日本血吸虫基因组内是否存在DNA重复序列。方法　分别根据曼氏血吸虫基因组特异的DNA重复序列单位Sm1- 7和埃及血吸虫基因组特异的DNA重复序列单位TheDraIRepeat设计并合成了寡核苷酸引物 ,然后以日本血吸虫基因组DNA为模板 ,用自备的多达 12种的反应缓冲液进行多聚酶链式反应 (PCR)优化扩增。结果　分别成功地从日本血吸虫基因组中扩增出与曼氏血吸虫基因组“特异的”DNA重复序列单位Sm1- 7和埃及血吸虫基因组“特异的”DNA重复序列单位TheDraIRepeat同源的DNA重复序列。PCR反应提示该两种重复序列在日本血吸虫基因组内的拷贝数均较低。同时 ,日本血吸虫与曼氏血吸虫表现出较好的同源性。结论　日本血吸虫基因组内可能存在着长度为 12 1bp的DNA重复序列 。Objective To determine whether there were any repetitive DNA sequences in the genome of Schistosoma japonicum.Methods Two sets of oligo-nucleotide primers were designed based on the repetitive DNA sequence unit in the genome of Schlstosoma mansoni and Schistosoma haematobium,respectively.Then,PCR amplifications were conducted with the genomic DNA of S.japonicum being the template.The PCR amplification was optimized using twelve PCR reaction buffers prepared in our laboratory,which proved to be highly efficient.Results Based on PCR optimization assays,repetitive DNA sequences similar to either one of these two repetitive DNA sequence units were successfully amplified (Sm1-7,specific to S.mansoni and the Dral repeat,specific to S.haemarobium).However,both repeats were much less presented in the genome of S.japonicum according to PCR assays.It was also noted that the S.mansoni-specific repetitive DNA sequence unit (Sm1-7) was better represented in the genome of S.japonicum than the S.haematobium-specific repetitive DNA sequence unit (the Dral repeat).Conclusion There might be a family of 121bp,repetitively represented DNA sequences in the genome of S.Japonicum.Further determination necessitated DNA sequence analysis.

关 键 词：多聚酶链式反应 DNA重复序列 血吸虫 基因组 

分 类 号：R383.24[医药卫生—医学寄生虫学] Q78[医药卫生—基础医学]