双峰驼诱导排卵机理的研究Ⅲ——双峰驼诱导排卵因子分布与代谢途径的研究  

Ovulation Inducing Mechanism in Bactrian Camel Ш Distribution and Metabolic Pathway of Ovulation Inducing Factor in Bactrian Camel

作　　者：潘光武[1] 汪静[2] 才学鹏[1] 刘湘涛[1] 邓敬兰[2] 谢庆阁[1] 潘元青[3] 石玉文 陈应福 

机构地区：[1]中国农业科学院兰州兽医研究所,甘肃兰州730046 [2]第四军医大学西京医院,陕西西安710032 [3]兰州医学院,甘肃兰州730002 [4]甘肃民勤县草原牧医站,733300

出　　处：《动物医学进展》2002年第6期96-100,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目 (3 92 70 5 3 4;3 9670 5 3 9);兰州兽医研究所所长基金资助项目

摘　　要：从公驼精清内经 DEAE-DE52 柱上分离的诱导排卵活性蛋白 (Ovulation-inducingbioactive protein,OIP)应用 12 5I标记后输入母驼阴道 ,通过血液循环到达垂体。经测定 ,OIP遍布母驼体内各脏器 ,其中以垂体、肝脏、心脏、舌肌等浓度较高 ,而在眼角膜、耳尖部以及被毛中含量很少。消除半衰期长达 72 .6 h,其代谢主要经肝胆系统排泄 ,也有部分活性蛋白通过泌尿。The ovulation inducing bioactive protein (OIP) from se minal plasma in male camel was isolated and iodinated with 125 I of isotope. Target of OIF was reached at pituitary gland passed blood brain barrier (BBB) and hypothalamus via the blood circulation after inse mination of OIP eluted on DEAE DE52 column. Distribution of OIP was detected in tissues and organs of the body of female.Results showed that highest levels of OIP were found in the pituitary,liver,Cardiac muscle and tongue as well as cornea of eye, tops of ears and fur in lower concentration. Eli mination period of half life within OIP was decreased in all of tissues and organs about 72.6hr after inse mination and mainly excreted in the intestine by the hepar bile circulation and secondary egested by the urinary system and external genital tracts.

关 键 词：双峰驼 机理 诱导排卵因子 分布 代谢途径 

分 类 号：S824.3[农业科学—畜牧学]