酶法测定血清中胆汁酸  

Enzymatic Method for the Determination of Bile Acids in Serum

作　　者：马晓航[1] 汪子伟[2] 贾小明[1] 王园园[1] 赵更峰[1] 

机构地区：[1]浙江大学生命科学院,杭州310029 [2]浙江省人民医院,杭州310014

出　　处：《分析化学》2002年第11期1289-1292,共4页Chinese Journal of Analytical Chemistry

摘　　要：研究了用 3 α羟基类固醇脱氢酶与黄递酶双酶体系催化的酶促胆汁酸测定方法。对影响酶促反应测定结果的主要因素进行了系统的研究 ,并在其基础上确定了测定试剂的组成为 :黄递酶 ,1 75U L;3 α羟基类固醇脱氢酶 60 0U L;烟酰胺腺嘌呤二核苷酸 (NAD+ ) 0 .88mmol L ;硝基四唑蓝 (NTB) 1 75mg L ;磷酸盐缓冲溶液 (pH 7.0 ) ,5 0mmol L。用以上试剂对胆汁酸测定的线性范围为 0～3 0 0 μmol L ,RSD为 3 .3 % ,回收率为1 0 5 .3 %。The main factors that may affect the result of the enzymatic method based on catalysis of 3-alpha-hydroxysteroid dehydrogenase and diaphorase system for the determination bile acid were studied. Experimental result shows that the conditions for the determination of bile acid are as follows: Diaphorase, 175 U/L; 3-alpha-hydroxysteroid dehydrogenase, 600 U/L; nicotinamide adenine dinucleotide (NAD(+)), 0.88 mmol/L; nitrotetrazolium blue (NTB), 175 mg/L; pH, 7.0. The linear range is 0 similar to 300 mumol/L, recovery is 105.3% and the RSD is 3.3%.

关 键 词：酶法测定 血清 胆汗酸 3-α羟基类固醇脱氢酶 黄递酶 肝脏 功能 临床检验 

分 类 号：R446.11[医药卫生—诊断学]